Cell-free DNA diagnostics in transplantation utilizing next generation sequencing.

Journal Article (Review;Journal Article)

The use of Next Generation Sequencing (NGS) to interrogate cell-free DNA (cfDNA) as a transplant diagnostic provides a crucial step in improving the accuracy of post-transplant monitoring of allograft health. cfDNA interrogation provides a powerful, yet minimally invasive, biomarker for disease and tissue injury. cfDNA can be isolated from a variety of body fluids and analyzed using bioinformatics to unlock its origins. Furthermore, cfDNA characteristics can reveal the mechanisms and conditions under which it was generated and released. In transplantation, donor-derived cfDNA monitoring provides a tool for identifying active allograft injury at the time of transplant, infection, and rejection. Multiple detection and interrogation methods for cfDNA detection are now being evaluated for clinical validity and hold the promise to provide minimally invasive, quantitative, and reproducible measures of allograft injury across organ types.

Full Text

Duke Authors

Cited Authors

  • Jackson, AM; Amato-Menker, C; Bettinotti, M

Published Date

  • November 2021

Published In

Volume / Issue

  • 82 / 11

Start / End Page

  • 850 - 858

PubMed ID

  • 34600770

Electronic International Standard Serial Number (EISSN)

  • 1879-1166

International Standard Serial Number (ISSN)

  • 0198-8859

Digital Object Identifier (DOI)

  • 10.1016/j.humimm.2021.07.006


  • eng