A bacterial kinase phosphorylates OSK1 to suppress stomatal immunity in rice.

Journal Article (Journal Article)

The Xanthomonas outer protein C2 (XopC2) family of bacterial effectors is widely found in plant pathogens and Legionella species. However, the biochemical activity and host targets of these effectors remain enigmatic. Here we show that ectopic expression of XopC2 promotes jasmonate signaling and stomatal opening in transgenic rice plants, which are more susceptible to Xanthomonas oryzae pv. oryzicola infection. Guided by these phenotypes, we discover that XopC2 represents a family of atypical kinases that specifically phosphorylate OSK1, a universal adaptor protein of the Skp1-Cullin-F-box ubiquitin ligase complexes. Intriguingly, OSK1 phosphorylation at Ser53 by XopC2 exclusively increases the binding affinity of OSK1 to the jasmonate receptor OsCOI1b, and specifically enhances the ubiquitination and degradation of JAZ transcription repressors and plant disease susceptibility through inhibiting stomatal immunity. These results define XopC2 as a prototypic member of a family of pathogenic effector kinases and highlight a smart molecular mechanism to activate jasmonate signaling.

Full Text

Duke Authors

Cited Authors

  • Wang, S; Li, S; Wang, J; Li, Q; Xin, X-F; Zhou, S; Wang, Y; Li, D; Xu, J; Luo, Z-Q; He, SY; Sun, W

Published Date

  • September 16, 2021

Published In

Volume / Issue

  • 12 / 1

Start / End Page

  • 5479 -

PubMed ID

  • 34531388

Pubmed Central ID

  • PMC8445998

Electronic International Standard Serial Number (EISSN)

  • 2041-1723

International Standard Serial Number (ISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/s41467-021-25748-4

Language

  • eng