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Human colon cancer cells express multiple glycoprotein ligands for E-selectin.

Publication ,  Journal Article
Tomlinson, J; Wang, JL; Barsky, SH; Lee, MC; Bischoff, J; Nguyen, M
Published in: Int J Oncol
February 2000

The interaction between the colon tumor cell surface and the endothelial cell layer is an important component of tumor intravasation, extravasation, and metastasis. Multiple studies suggest that tumor cells may bind to E-selectin expressed on endothelial cells during these processes. To identify possible E-selectin ligands on tumor cells that may participate in this mechanism, we used E-selectin-Ig chimera affinity chromatography to isolate glycoproteins from the human colon cancer cell line Colo-205. Binding of these cells to E-selectin was specific, required the presence of calcium, and could be blocked by antibodies against E-selectin. We identified LAMP-1 (lysosomal membrane glycoprotein-1), LAMP-2, and two high molecular weight glycoproteins (>400 kDa and 300 kDa) as the main E-selectin ligands on Colo-205 cells. Treatment of the cells with N-glycanase and O-sialoglycoprotease abolished their binding to E-selectin. The high MW glycoproteins contained sialyl Lewis X and/or sialyl Lewis A glycoconjugates, and appeared to be either alternatively spliced or alternatively glycosylated forms of MUC-1 (mucin-1).

Duke Scholars

Published In

Int J Oncol

DOI

ISSN

1019-6439

Publication Date

February 2000

Volume

16

Issue

2

Start / End Page

347 / 353

Location

Greece

Related Subject Headings

  • Tumor Cells, Cultured
  • Recombinant Fusion Proteins
  • Oncology & Carcinogenesis
  • Neoplasm Proteins
  • Mice
  • Membrane Glycoproteins
  • Lysosomal Membrane Proteins
  • Ligands
  • Humans
  • Glycoproteins
 

Citation

APA
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Tomlinson, J., Wang, J. L., Barsky, S. H., Lee, M. C., Bischoff, J., & Nguyen, M. (2000). Human colon cancer cells express multiple glycoprotein ligands for E-selectin. Int J Oncol, 16(2), 347–353. https://doi.org/10.3892/ijo.16.2.347
Tomlinson, J., J. L. Wang, S. H. Barsky, M. C. Lee, J. Bischoff, and M. Nguyen. “Human colon cancer cells express multiple glycoprotein ligands for E-selectin.Int J Oncol 16, no. 2 (February 2000): 347–53. https://doi.org/10.3892/ijo.16.2.347.
Tomlinson J, Wang JL, Barsky SH, Lee MC, Bischoff J, Nguyen M. Human colon cancer cells express multiple glycoprotein ligands for E-selectin. Int J Oncol. 2000 Feb;16(2):347–53.
Tomlinson, J., et al. “Human colon cancer cells express multiple glycoprotein ligands for E-selectin.Int J Oncol, vol. 16, no. 2, Feb. 2000, pp. 347–53. Pubmed, doi:10.3892/ijo.16.2.347.
Tomlinson J, Wang JL, Barsky SH, Lee MC, Bischoff J, Nguyen M. Human colon cancer cells express multiple glycoprotein ligands for E-selectin. Int J Oncol. 2000 Feb;16(2):347–353.

Published In

Int J Oncol

DOI

ISSN

1019-6439

Publication Date

February 2000

Volume

16

Issue

2

Start / End Page

347 / 353

Location

Greece

Related Subject Headings

  • Tumor Cells, Cultured
  • Recombinant Fusion Proteins
  • Oncology & Carcinogenesis
  • Neoplasm Proteins
  • Mice
  • Membrane Glycoproteins
  • Lysosomal Membrane Proteins
  • Ligands
  • Humans
  • Glycoproteins