Transcriptome-Wide Identification of 2'-O-Methylation Sites with RibOxi-Seq.

Journal Article (Journal Article)

The ability to detect 2'-O-methylation sites (Nm) in high-throughput fashion is important, as increasing evidence points to a more diverse landscape for this RNA modification as well as the possibility of yet unidentified functions. Here we describe an optimized version of RibOxi-seq, which is built upon the original published method, that not only accurately profiles ribosomal RNA (rRNA) Nm sites with minimal RNA input but is also robust enough to identify mRNA intronic and exonic sites.

Full Text

Duke Authors

Cited Authors

  • Zhu, Y; Holley, CL; Carmichael, GG

Published Date

  • 2022

Published In

Volume / Issue

  • 2404 /

Start / End Page

  • 393 - 407

PubMed ID

  • 34694622

Pubmed Central ID

  • PMC9165532

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-0716-1851-6_22

Language

  • eng

Conference Location

  • United States