Transcriptome-Wide Identification of 2'-O-Methylation Sites with RibOxi-Seq.
Publication
, Journal Article
Zhu, Y; Holley, CL; Carmichael, GG
Published in: Methods Mol Biol
2022
The ability to detect 2'-O-methylation sites (Nm) in high-throughput fashion is important, as increasing evidence points to a more diverse landscape for this RNA modification as well as the possibility of yet unidentified functions. Here we describe an optimized version of RibOxi-seq, which is built upon the original published method, that not only accurately profiles ribosomal RNA (rRNA) Nm sites with minimal RNA input but is also robust enough to identify mRNA intronic and exonic sites.
Duke Scholars
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Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2022
Volume
2404
Start / End Page
393 / 407
Location
United States
Related Subject Headings
- Transcriptome
- RNA, Ribosomal
- RNA
- Methylation
- Developmental Biology
- Base Sequence
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Zhu, Y., Holley, C. L., & Carmichael, G. G. (2022). Transcriptome-Wide Identification of 2'-O-Methylation Sites with RibOxi-Seq. Methods Mol Biol, 2404, 393–407. https://doi.org/10.1007/978-1-0716-1851-6_22
Zhu, Yinzhou, Christopher L. Holley, and Gordon G. Carmichael. “Transcriptome-Wide Identification of 2'-O-Methylation Sites with RibOxi-Seq.” Methods Mol Biol 2404 (2022): 393–407. https://doi.org/10.1007/978-1-0716-1851-6_22.
Zhu Y, Holley CL, Carmichael GG. Transcriptome-Wide Identification of 2'-O-Methylation Sites with RibOxi-Seq. Methods Mol Biol. 2022;2404:393–407.
Zhu, Yinzhou, et al. “Transcriptome-Wide Identification of 2'-O-Methylation Sites with RibOxi-Seq.” Methods Mol Biol, vol. 2404, 2022, pp. 393–407. Pubmed, doi:10.1007/978-1-0716-1851-6_22.
Zhu Y, Holley CL, Carmichael GG. Transcriptome-Wide Identification of 2'-O-Methylation Sites with RibOxi-Seq. Methods Mol Biol. 2022;2404:393–407.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2022
Volume
2404
Start / End Page
393 / 407
Location
United States
Related Subject Headings
- Transcriptome
- RNA, Ribosomal
- RNA
- Methylation
- Developmental Biology
- Base Sequence
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences