Acoustofluidic Droplet Sorter Based on Single Phase Focused Transducers.

Journal Article (Journal Article)

Droplet microfluidics has revolutionized the biomedical and drug development fields by allowing for independent microenvironments to conduct drug screening at the single cell level. However, current microfluidic sorting devices suffer from drawbacks such as high voltage requirements (e.g., >200 Vpp), low biocompatibility, and/or low throughput. In this article, a single-phase focused transducer (SPFT)-based acoustofluidic chip is introduced, which outperforms many microfluidic droplet sorting devices through high energy transmission efficiency, high accuracy, and high biocompatibility. The SPFT-based sorter can be driven with an input power lower than 20 Vpp and maintain a postsorting cell viability of 93.5%. The SPFT sorter can achieve a throughput over 1000 events per second and a sorting purity up to 99.2%. The SPFT sorter is utilized here for the screening of doxorubicin cytotoxicity on cancer and noncancer cells, proving its drug screening capability. Overall, the SPFT droplet sorting device shows great potential for fast, precise, and biocompatible drug screening.

Full Text

Duke Authors

Cited Authors

  • Zhong, R; Yang, S; Ugolini, GS; Naquin, T; Zhang, J; Yang, K; Xia, J; Konry, T; Huang, TJ

Published Date

  • October 17, 2021

Published In

Start / End Page

  • e2103848 -

PubMed ID

  • 34658129

Pubmed Central ID

  • PMC8686687

Electronic International Standard Serial Number (EISSN)

  • 1613-6829

International Standard Serial Number (ISSN)

  • 1613-6810

Digital Object Identifier (DOI)

  • 10.1002/smll.202103848

Language

  • eng