Evidence for nutrient-dependent regulation of the COPII coat by O-GlcNAcylation.

Journal Article (Journal Article)

O-linked β-N-acetylglucosamine (O-GlcNAc) is a dynamic form of intracellular glycosylation common in animals, plants and other organisms. O-GlcNAcylation is essential in mammalian cells and is dysregulated in myriad human diseases, such as cancer, neurodegeneration and metabolic syndrome. Despite this pathophysiological significance, key aspects of O-GlcNAc signaling remain incompletely understood, including its impact on fundamental cell biological processes. Here, we investigate the role of O-GlcNAcylation in the coat protein II complex (COPII), a system universally conserved in eukaryotes that mediates anterograde vesicle trafficking from the endoplasmic reticulum. We identify new O-GlcNAcylation sites on Sec24C, Sec24D and Sec31A, core components of the COPII system, and provide evidence for potential nutrient-sensitive pathway regulation through site-specific glycosylation. Our work suggests a new connection between metabolism and trafficking through the conduit of COPII protein O-GlcNAcylation.

Full Text

Duke Authors

Cited Authors

  • Bisnett, BJ; Condon, BM; Linhart, NA; Lamb, CH; Huynh, DT; Bai, J; Smith, TJ; Hu, J; Georgiou, GR; Boyce, M

Published Date

  • September 20, 2021

Published In

Volume / Issue

  • 31 / 9

Start / End Page

  • 1102 - 1120

PubMed ID

  • 34142147

Pubmed Central ID

  • PMC8457363

Electronic International Standard Serial Number (EISSN)

  • 1460-2423

Digital Object Identifier (DOI)

  • 10.1093/glycob/cwab055

Language

  • eng

Conference Location

  • England