Cre-dependent adeno-associated virus preparation and delivery for labeling neurons in the mouse brain.

Journal Article (Journal Article)

Virus-mediated gene delivery is a powerful strategy for labeling and manipulating neurons in mammalian brains. A major drawback of this gene delivery method has been the lack of cell-type specificity. However, methods that combine Cre-knockin mice and Cre-activated adeno-associated virus (AAV) have now been developed to achieve high-level, stable, and cell-type-specific gene expression. Here, we describe a protocol for the design, production, and delivery of Cre-dependent AAVs. This method is simple and highly efficient, and allows chronic live imaging of defined classes of synapses in the mouse brain.

Full Text

Duke Authors

Cited Authors

  • Huang, ZJ; Taniguchi, H; He, M; Kuhlman, S

Published Date

  • February 1, 2014

Published In

Volume / Issue

  • 2014 / 2

Start / End Page

  • 190 - 194

PubMed ID

  • 24492777

Electronic International Standard Serial Number (EISSN)

  • 1559-6095

Digital Object Identifier (DOI)

  • 10.1101/pdb.prot080382

Language

  • eng

Conference Location

  • United States