A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale.

Journal Article (Journal Article)

In this era of complete genomes, our knowledge of neuroanatomical circuitry remains surprisingly sparse. Such knowledge is critical, however, for both basic and clinical research into brain function. Here we advocate for a concerted effort to fill this gap, through systematic, experimental mapping of neural circuits at a mesoscopic scale of resolution suitable for comprehensive, brainwide coverage, using injections of tracers or viral vectors. We detail the scientific and medical rationale and briefly review existing knowledge and experimental techniques. We define a set of desiderata, including brainwide coverage; validated and extensible experimental techniques suitable for standardization and automation; centralized, open-access data repository; compatibility with existing resources; and tractability with current informatics technology. We discuss a hypothetical but tractable plan for mouse, additional efforts for the macaque, and technique development for human. We estimate that the mouse connectivity project could be completed within five years with a comparatively modest budget.

Full Text

Duke Authors

Cited Authors

  • Bohland, JW; Wu, C; Barbas, H; Bokil, H; Bota, M; Breiter, HC; Cline, HT; Doyle, JC; Freed, PJ; Greenspan, RJ; Haber, SN; Hawrylycz, M; Herrera, DG; Hilgetag, CC; Huang, ZJ; Jones, A; Jones, EG; Karten, HJ; Kleinfeld, D; Kötter, R; Lester, HA; Lin, JM; Mensh, BD; Mikula, S; Panksepp, J; Price, JL; Safdieh, J; Saper, CB; Schiff, ND; Schmahmann, JD; Stillman, BW; Svoboda, K; Swanson, LW; Toga, AW; Van Essen, DC; Watson, JD; Mitra, PP

Published Date

  • March 2009

Published In

Volume / Issue

  • 5 / 3

Start / End Page

  • e1000334 -

PubMed ID

  • 19325892

Pubmed Central ID

  • PMC2655718

Electronic International Standard Serial Number (EISSN)

  • 1553-7358

Digital Object Identifier (DOI)

  • 10.1371/journal.pcbi.1000334


  • eng

Conference Location

  • United States