SLC1A5 provides glutamine and asparagine necessary for bone development in mice.

Journal Article (Journal Article)

Osteoblast differentiation is sequentially characterized by high rates of proliferation followed by increased protein and matrix synthesis, processes that require substantial amino acid acquisition and production. How osteoblasts obtain or maintain intracellular amino acid production is poorly understood. Here, we identify SLC1A5 as a critical amino acid transporter during bone development. Using a genetic and metabolomic approach, we show SLC1A5 acts cell autonomously to regulate protein synthesis and osteoblast differentiation. SLC1A5 provides both glutamine and asparagine which are essential for osteoblast differentiation. Mechanistically, glutamine and to a lesser extent asparagine support amino acid biosynthesis. Thus, osteoblasts depend on Slc1a5 to provide glutamine and asparagine, which are subsequently used to produce non-essential amino acids and support osteoblast differentiation and bone development.

Full Text

Duke Authors

Cited Authors

  • Sharma, D; Yu, Y; Shen, L; Zhang, G-F; Karner, CM

Published Date

  • October 14, 2021

Published In

Volume / Issue

  • 10 /

PubMed ID

  • 34647520

Pubmed Central ID

  • PMC8553342

Electronic International Standard Serial Number (EISSN)

  • 2050-084X

Digital Object Identifier (DOI)

  • 10.7554/eLife.71595

Language

  • eng

Conference Location

  • England