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Combining Nelfinavir With Chloroquine Inhibits In Vivo Growth of Human Lung Cancer Xenograft Tumors.

Publication ,  Journal Article
Lopiccolo, J; Kawabata, S; Gills, JJ; Dennis, PA
Published in: In Vivo
2021

BACKGROUND/AIM: Nelfinavir is a human immunodeficiency virus protease inhibitor that is currently being repositioned as an anticancer drug. Chloroquine, an anti-malarial lysosomotropic drug, inhibits autophagy. It has been reported that the combination of nelfinavir and chloroquine significantly enhances endoplasmic reticulum (ER) stress and induces selective cell death in multiple cell line models (in vitro). MATERIALS AND METHODS: We assessed the effects of the combination of these drugs on human NSCLC cell lines in vitro using cell proliferation assay and performed preclinical treatment studies using cell line-derived xenograft mouse models in vivo. RESULTS: In vitro, this combination enhanced inhibition of NSCLC cell proliferation with increased proteotoxicity, including ER stress, and apoptosis. In vivo, the growth of human NSCLC xenograft tumors was inhibited, which correlated with increased apoptosis and induction of ER stress as well as NSCLC growth in vitro. CONCLUSION: Our findings suggest that the induction of proteotoxicity provides a promising new target for developing anticancer drugs.

Duke Scholars

Published In

In Vivo

DOI

EISSN

1791-7549

Publication Date

2021

Volume

35

Issue

1

Start / End Page

141 / 145

Location

Greece

Related Subject Headings

  • Xenograft Model Antitumor Assays
  • Oncology & Carcinogenesis
  • Nelfinavir
  • Lung Neoplasms
  • Humans
  • Heterografts
  • Chloroquine
  • Cell Proliferation
  • Cell Line, Tumor
  • Apoptosis
 

Citation

APA
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ICMJE
MLA
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Lopiccolo, J., Kawabata, S., Gills, J. J., & Dennis, P. A. (2021). Combining Nelfinavir With Chloroquine Inhibits In Vivo Growth of Human Lung Cancer Xenograft Tumors. In Vivo, 35(1), 141–145. https://doi.org/10.21873/invivo.12241
Lopiccolo, Jaclyn, Shigeru Kawabata, Joell J. Gills, and Phillip A. Dennis. “Combining Nelfinavir With Chloroquine Inhibits In Vivo Growth of Human Lung Cancer Xenograft Tumors.In Vivo 35, no. 1 (2021): 141–45. https://doi.org/10.21873/invivo.12241.
Lopiccolo J, Kawabata S, Gills JJ, Dennis PA. Combining Nelfinavir With Chloroquine Inhibits In Vivo Growth of Human Lung Cancer Xenograft Tumors. In Vivo. 2021;35(1):141–5.
Lopiccolo, Jaclyn, et al. “Combining Nelfinavir With Chloroquine Inhibits In Vivo Growth of Human Lung Cancer Xenograft Tumors.In Vivo, vol. 35, no. 1, 2021, pp. 141–45. Pubmed, doi:10.21873/invivo.12241.
Lopiccolo J, Kawabata S, Gills JJ, Dennis PA. Combining Nelfinavir With Chloroquine Inhibits In Vivo Growth of Human Lung Cancer Xenograft Tumors. In Vivo. 2021;35(1):141–145.

Published In

In Vivo

DOI

EISSN

1791-7549

Publication Date

2021

Volume

35

Issue

1

Start / End Page

141 / 145

Location

Greece

Related Subject Headings

  • Xenograft Model Antitumor Assays
  • Oncology & Carcinogenesis
  • Nelfinavir
  • Lung Neoplasms
  • Humans
  • Heterografts
  • Chloroquine
  • Cell Proliferation
  • Cell Line, Tumor
  • Apoptosis