Large Volume, Behaviorally-relevant Illumination for Optogenetics in Non-human Primates.

Journal Article (Journal Article)

This protocol describes a large-volume illuminator, which was developed for optogenetic manipulations in the non-human primate brain. The illuminator is a modified plastic optical fiber with etched tip, such that the light emitting surface area is > 100x that of a conventional fiber. In addition to describing the construction of the large-volume illuminator, this protocol details the quality-control calibration used to ensure even light distribution. Further, this protocol describes techniques for inserting and removing the large volume illuminator. Both superficial and deep structures may be illuminated. This large volume illuminator does not need to be physically coupled to an electrode, and because the illuminator is made of plastic, not glass, it will simply bend in circumstances when traditional optical fibers would shatter. Because this illuminator delivers light over behaviorally-relevant tissue volumes (≈ 10 mm3) with no greater penetration damage than a conventional optical fiber, it facilitates behavioral studies using optogenetics in non-human primates.

Full Text

Duke Authors

Cited Authors

  • Acker, LC; Pino, EN; Boyden, ES; Desimone, R

Published Date

  • October 3, 2017

Published In

PubMed ID

  • 28994798

Pubmed Central ID

  • PMC5752367

Electronic International Standard Serial Number (EISSN)

  • 1940-087X

Digital Object Identifier (DOI)

  • 10.3791/56330

Language

  • eng

Conference Location

  • United States