Noninvasive optical inhibition with a red-shifted microbial rhodopsin.

Journal Article (Journal Article)

Optogenetic inhibition of the electrical activity of neurons enables the causal assessment of their contributions to brain functions. Red light penetrates deeper into tissue than other visible wavelengths. We present a red-shifted cruxhalorhodopsin, Jaws, derived from Haloarcula (Halobacterium) salinarum (strain Shark) and engineered to result in red light-induced photocurrents three times those of earlier silencers. Jaws exhibits robust inhibition of sensory-evoked neural activity in the cortex and results in strong light responses when used in retinas of retinitis pigmentosa model mice. We also demonstrate that Jaws can noninvasively mediate transcranial optical inhibition of neurons deep in the brains of awake mice. The noninvasive optogenetic inhibition opened up by Jaws enables a variety of important neuroscience experiments and offers a powerful general-use chloride pump for basic and applied neuroscience.

Full Text

Duke Authors

Cited Authors

  • Chuong, AS; Miri, ML; Busskamp, V; Matthews, GAC; Acker, LC; Sørensen, AT; Young, A; Klapoetke, NC; Henninger, MA; Kodandaramaiah, SB; Ogawa, M; Ramanlal, SB; Bandler, RC; Allen, BD; Forest, CR; Chow, BY; Han, X; Lin, Y; Tye, KM; Roska, B; Cardin, JA; Boyden, ES

Published Date

  • August 2014

Published In

Volume / Issue

  • 17 / 8

Start / End Page

  • 1123 - 1129

PubMed ID

  • 24997763

Pubmed Central ID

  • PMC4184214

Electronic International Standard Serial Number (EISSN)

  • 1546-1726

Digital Object Identifier (DOI)

  • 10.1038/nn.3752


  • eng

Conference Location

  • United States