A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines.

Journal Article (Journal Article)

Multiplex immunoassays with acellular antigens are well-established based on solid-phase platforms such as the Luminex® technology. Cell barcoding by amine-reactive fluorescent dyes enables analogous cell-based multiplex assays, but requires multiple labeling reactions and quality checks prior to every assay. Here we describe generation of stable, fluorescent protein-barcoded reporter cell lines suitable for multiplex screening of antibody to membrane proteins. The utility of this cell-based system, with the potential of a 256-plex cell panel, is demonstrated by flow cytometry deconvolution of barcoded cell panels expressing influenza A hemagglutinin trimers, or native human CCR2 or CCR5 multi-span proteins and their epitope-defining mutants. This platform will prove useful for characterizing immunity and discovering antibodies to membrane-associated proteins.

Full Text

Duke Authors

Cited Authors

  • Song, S; Manook, M; Kwun, J; Jackson, AM; Knechtle, SJ; Kelsoe, G

Published Date

  • November 25, 2021

Published In

Volume / Issue

  • 4 / 1

Start / End Page

  • 1338 -

PubMed ID

  • 34824350

Pubmed Central ID

  • PMC8617053

Electronic International Standard Serial Number (EISSN)

  • 2399-3642

Digital Object Identifier (DOI)

  • 10.1038/s42003-021-02881-w


  • eng

Conference Location

  • England