Rapid test to assess the escape of SARS-CoV-2 variants of concern.

Journal Article (Journal Article)

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants are concerning in the ongoing coronavirus disease 2019 (COVID-19) pandemic. Here, we developed a rapid test, termed CoVariant-SCAN, that detects neutralizing antibodies (nAbs) capable of blocking interactions between the angiotensin-converting enzyme 2 receptor and the spike protein of wild-type (WT) SARS-CoV-2 and three other variants: B.1.1.7, B.1.351, and P.1. Using CoVariant-SCAN, we assessed neutralization/blocking of monoclonal antibodies and plasma from COVID-19–positive and vaccinated individuals. For several monoclonal antibodies and most plasma samples, neutralization against B.1.351 and P.1 variants is diminished relative to WT, while B.1.1.7 is largely cross-neutralized. We also showed that we can rapidly adapt the platform to detect nAbs against an additional variant—B.1.617.2 (Delta)—without reengineering or reoptimizing the assay. Results using CoVariant-SCAN are consistent with live virus neutralization assays and demonstrate that this easy-to-deploy test could be used to rapidly assess nAb response against multiple SARS-CoV-2 variants.

Full Text

Duke Authors

Cited Authors

  • Heggestad, JT; Britton, RJ; Kinnamon, DS; Wall, SA; Joh, DY; Hucknall, AM; Olson, LB; Anderson, JG; Mazur, A; Wolfe, CR; Oguin, TH; Sullenger, BA; Burke, TW; Kraft, BD; Sempowski, GD; Woods, CW; Chilkoti, A

Published Date

  • December 3, 2021

Published In

Volume / Issue

  • 7 / 49

Start / End Page

  • eabl7682 -

PubMed ID

  • 34860546

Pubmed Central ID

  • PMC8641938

Electronic International Standard Serial Number (EISSN)

  • 2375-2548

Digital Object Identifier (DOI)

  • 10.1126/sciadv.abl7682


  • eng

Conference Location

  • United States