Skip to main content

Smartphone-Based Device for Colorimetric Detection of MicroRNA Biomarkers Using Nanoparticle-Based Assay.

Publication ,  Journal Article
Krishnan, T; Wang, H-N; Vo-Dinh, T
Published in: Sensors (Basel, Switzerland)
December 2021

The detection of microRNAs (miRNAs) is emerging as a clinically important tool for the non-invasive detection of a wide variety of diseases ranging from cancers and cardiovascular illnesses to infectious diseases. Over the years, miRNA detection schemes have become accessible to clinicians, but they still require sophisticated and bulky laboratory equipment and trained personnel to operate. The exceptional computing ability and ease of use of modern smartphones coupled with fieldable optical detection technologies can provide a useful and portable alternative to these laboratory systems. Herein, we present the development of a smartphone-based device called Krometriks, which is capable of simple and rapid colorimetric detection of microRNA (miRNAs) using a nanoparticle-based assay. The device consists of a smartphone, a 3D printed accessory, and a custom-built dedicated mobile app. We illustrate the utility of Krometriks for the detection of an important miRNA disease biomarker, miR-21, using a nanoplasmonics-based assay developed by our group. We show that Krometriks can detect miRNA down to nanomolar concentrations with detection results comparable to a laboratory-based benchtop spectrophotometer. With slight changes to the accessory design, Krometriks can be made compatible with different types of smartphone models and specifications. Thus, the Krometriks device offers a practical colorimetric platform that has the potential to provide accessible and affordable miRNA diagnostics for point-of-care and field applications in low-resource settings.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Sensors (Basel, Switzerland)

DOI

EISSN

1424-8220

ISSN

1424-8220

Publication Date

December 2021

Volume

21

Issue

23

Start / End Page

8044

Related Subject Headings

  • Smartphone
  • Nanoparticles
  • MicroRNAs
  • Colorimetry
  • Biomarkers
  • Analytical Chemistry
  • 4606 Distributed computing and systems software
  • 4104 Environmental management
  • 4009 Electronics, sensors and digital hardware
  • 4008 Electrical engineering
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Krishnan, T., Wang, H.-N., & Vo-Dinh, T. (2021). Smartphone-Based Device for Colorimetric Detection of MicroRNA Biomarkers Using Nanoparticle-Based Assay. Sensors (Basel, Switzerland), 21(23), 8044. https://doi.org/10.3390/s21238044
Krishnan, Tushar, Hsin-Neng Wang, and Tuan Vo-Dinh. “Smartphone-Based Device for Colorimetric Detection of MicroRNA Biomarkers Using Nanoparticle-Based Assay.Sensors (Basel, Switzerland) 21, no. 23 (December 2021): 8044. https://doi.org/10.3390/s21238044.
Krishnan T, Wang H-N, Vo-Dinh T. Smartphone-Based Device for Colorimetric Detection of MicroRNA Biomarkers Using Nanoparticle-Based Assay. Sensors (Basel, Switzerland). 2021 Dec;21(23):8044.
Krishnan, Tushar, et al. “Smartphone-Based Device for Colorimetric Detection of MicroRNA Biomarkers Using Nanoparticle-Based Assay.Sensors (Basel, Switzerland), vol. 21, no. 23, Dec. 2021, p. 8044. Epmc, doi:10.3390/s21238044.
Krishnan T, Wang H-N, Vo-Dinh T. Smartphone-Based Device for Colorimetric Detection of MicroRNA Biomarkers Using Nanoparticle-Based Assay. Sensors (Basel, Switzerland). 2021 Dec;21(23):8044.

Published In

Sensors (Basel, Switzerland)

DOI

EISSN

1424-8220

ISSN

1424-8220

Publication Date

December 2021

Volume

21

Issue

23

Start / End Page

8044

Related Subject Headings

  • Smartphone
  • Nanoparticles
  • MicroRNAs
  • Colorimetry
  • Biomarkers
  • Analytical Chemistry
  • 4606 Distributed computing and systems software
  • 4104 Environmental management
  • 4009 Electronics, sensors and digital hardware
  • 4008 Electrical engineering