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Evaluation of the interaction between insecticide resistance-associated genes and malaria transmission in Anopheles gambiae sensu lato in central Côte d'Ivoire.

Publication ,  Journal Article
Wolie, RZ; Koffi, AA; Ahoua Alou, LP; Sternberg, ED; N'Nan-Alla, O; Dahounto, A; Yapo, FHA; Kanh, KMH; Camara, S; Oumbouke, WA; Tia, IZ ...
Published in: Parasit Vectors
November 20, 2021

BACKGROUND: There is evidence that the knockdown resistance gene (Kdr) L1014F and acetylcholinesterase-1 gene (Ace-1R) G119S mutations involved in pyrethroid and carbamate resistance in Anopheles gambiae influence malaria transmission in sub-Saharan Africa. This is likely due to changes in the behaviour, life history and vector competence and capacity of An. gambiae. In the present study, performed as part of a two-arm cluster randomized controlled trial evaluating the impact of household screening plus a novel insecticide delivery system (In2Care Eave Tubes), we investigated the distribution of insecticide target site mutations and their association with infection status in wild An. gambiae sensu lato (s.l.) populations. METHODS: Mosquitoes were captured in 40 villages around Bouaké by human landing catch from May 2017 to April 2019. Randomly selected samples of An. gambiae s.l. that were infected or not infected with Plasmodium sp. were identified to species and then genotyped for Kdr L1014F and Ace-1R G119S mutations using quantitative polymerase chain reaction assays. The frequencies of the two alleles were compared between Anopheles coluzzii and Anopheles gambiae and then between infected and uninfected groups for each species. RESULTS: The presence of An. gambiae (49%) and An. coluzzii (51%) was confirmed in Bouaké. Individuals of both species infected with Plasmodium parasites were found. Over the study period, the average frequency of the Kdr L1014F and Ace-1R G119S mutations did not vary significantly between study arms. However, the frequencies of the Kdr L1014F and Ace-1R G119S resistance alleles were significantly higher in An. gambiae than in An. coluzzii [odds ratio (95% confidence interval): 59.64 (30.81-131.63) for Kdr, and 2.79 (2.17-3.60) for Ace-1R]. For both species, there were no significant differences in Kdr L1014F or Ace-1R G119S genotypic and allelic frequency distributions between infected and uninfected specimens (P > 0.05). CONCLUSIONS: Either alone or in combination, Kdr L1014F and Ace-1R G119S showed no significant association with Plasmodium infection in wild An. gambiae and An. coluzzii, demonstrating the similar competence of these species for Plasmodium transmission in Bouaké. Additional factors including behavioural and environmental ones that influence vector competence in natural populations, and those other than allele measurements (metabolic resistance factors) that contribute to resistance, should be considered when establishing the existence of a link between insecticide resistance and vector competence.

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Published In

Parasit Vectors

DOI

EISSN

1756-3305

Publication Date

November 20, 2021

Volume

14

Issue

1

Start / End Page

581

Location

England

Related Subject Headings

  • Tropical Medicine
  • Plasmodium
  • Mycology & Parasitology
  • Mosquito Vectors
  • Mosquito Control
  • Malaria
  • Insecticides
  • Insecticide Resistance
  • Genes, Insect
  • Cote d'Ivoire
 

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Wolie, R. Z., Koffi, A. A., Ahoua Alou, L. P., Sternberg, E. D., N’Nan-Alla, O., Dahounto, A., … NGuessan, R. (2021). Evaluation of the interaction between insecticide resistance-associated genes and malaria transmission in Anopheles gambiae sensu lato in central Côte d'Ivoire. Parasit Vectors, 14(1), 581. https://doi.org/10.1186/s13071-021-05079-5
Wolie, Rosine Z., Alphonsine A. Koffi, Ludovic P. Ahoua Alou, Eleanore D. Sternberg, Oulo N’Nan-Alla, Amal Dahounto, Florent H. A. Yapo, et al. “Evaluation of the interaction between insecticide resistance-associated genes and malaria transmission in Anopheles gambiae sensu lato in central Côte d'Ivoire.Parasit Vectors 14, no. 1 (November 20, 2021): 581. https://doi.org/10.1186/s13071-021-05079-5.
Wolie RZ, Koffi AA, Ahoua Alou LP, Sternberg ED, N’Nan-Alla O, Dahounto A, et al. Evaluation of the interaction between insecticide resistance-associated genes and malaria transmission in Anopheles gambiae sensu lato in central Côte d'Ivoire. Parasit Vectors. 2021 Nov 20;14(1):581.
Wolie, Rosine Z., et al. “Evaluation of the interaction between insecticide resistance-associated genes and malaria transmission in Anopheles gambiae sensu lato in central Côte d'Ivoire.Parasit Vectors, vol. 14, no. 1, Nov. 2021, p. 581. Pubmed, doi:10.1186/s13071-021-05079-5.
Wolie RZ, Koffi AA, Ahoua Alou LP, Sternberg ED, N’Nan-Alla O, Dahounto A, Yapo FHA, Kanh KMH, Camara S, Oumbouke WA, Tia IZ, Nguetta S-PA, Thomas MB, NGuessan R. Evaluation of the interaction between insecticide resistance-associated genes and malaria transmission in Anopheles gambiae sensu lato in central Côte d'Ivoire. Parasit Vectors. 2021 Nov 20;14(1):581.
Journal cover image

Published In

Parasit Vectors

DOI

EISSN

1756-3305

Publication Date

November 20, 2021

Volume

14

Issue

1

Start / End Page

581

Location

England

Related Subject Headings

  • Tropical Medicine
  • Plasmodium
  • Mycology & Parasitology
  • Mosquito Vectors
  • Mosquito Control
  • Malaria
  • Insecticides
  • Insecticide Resistance
  • Genes, Insect
  • Cote d'Ivoire