Trav15-dv6 family Tcrd rearrangements diversify the Tcra repertoire.

Journal Article (Journal Article)

The Tcra repertoire is generated by multiple rounds of Vα-Jα rearrangement. However, Tcrd recombination precedes Tcra recombination within the complex Tcra-Tcrd locus. Here, by ablating Tcrd recombination, we report that Tcrd rearrangement broadens primary Vα use to diversify the Tcra repertoire in mice. We reveal that use of Trav15-dv6 family V gene segments in Tcrd recombination imparts diversity in the Tcra repertoire by instigating use of central and distal Vα segments. Moreover, disruption of the regions containing these genes and their cis-regulatory elements identifies the Trav15-dv6 family as being responsible for driving central and distal Vα recombinations beyond their roles as substrates for Tcrd recombination. Our study demonstrates an indispensable role for Tcrd recombination in general, and the Trav15-dv6 family in particular, in the generation of a combinatorially diverse Tcra repertoire.

Full Text

Duke Authors

Cited Authors

  • Dauphars, DJ; Mihai, A; Wang, L; Zhuang, Y; Krangel, MS

Published Date

  • February 7, 2022

Published In

Volume / Issue

  • 219 / 2

PubMed ID

  • 34910107

Pubmed Central ID

  • PMC8679779

Electronic International Standard Serial Number (EISSN)

  • 1540-9538

Digital Object Identifier (DOI)

  • 10.1084/jem.20211581


  • eng

Conference Location

  • United States