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KIF18B is a cell type-specific regulator of spindle orientation in the epidermis.

Publication ,  Journal Article
Moreci, RS; Lechler, T
Published in: Mol Biol Cell
November 1, 2021

Proper spindle orientation is required for asymmetric cell division and the establishment of complex tissue architecture. In the developing epidermis, spindle orientation requires a conserved cortical protein complex of LGN/NuMA/dynein-dynactin. However, how microtubule dynamics are regulated to interact with this machinery and properly position the mitotic spindle is not fully understood. Furthermore, our understanding of the processes that link spindle orientation during asymmetric cell division to cell fate specification in distinct tissue contexts remains incomplete. We report a role for the microtubule catastrophe factor KIF18B in regulating microtubule dynamics to promote spindle orientation in keratinocytes. During mitosis, KIF18B accumulates at the cell cortex, colocalizing with the conserved spindle orientation machinery. In vivo we find that KIF18B is required for oriented cell divisions within the hair placode, the first stage of hair follicle morphogenesis, but is not essential in the interfollicular epidermis. Disrupting spindle orientation in the placode, using mutations in either KIF18B or NuMA, results in aberrant cell fate marker expression of hair follicle progenitor cells. These data functionally link spindle orientation to cell fate decisions during hair follicle morphogenesis. Taken together, our data demonstrate a role for regulated microtubule dynamics in spindle orientation in epidermal cells. This work also highlights the importance of spindle orientation during asymmetric cell division to dictate cell fate specification.

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Published In

Mol Biol Cell

DOI

EISSN

1939-4586

Publication Date

November 1, 2021

Volume

32

Issue

21

Start / End Page

ar29

Location

United States

Related Subject Headings

  • Spindle Apparatus
  • Primary Cell Culture
  • Mitosis
  • Microtubules
  • Mice, Knockout
  • Mice
  • Male
  • Kinesins
  • Keratinocytes
  • Female
 

Citation

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Moreci, R. S., & Lechler, T. (2021). KIF18B is a cell type-specific regulator of spindle orientation in the epidermis. Mol Biol Cell, 32(21), ar29. https://doi.org/10.1091/mbc.E21-06-0291
Moreci, Rebecca S., and Terry Lechler. “KIF18B is a cell type-specific regulator of spindle orientation in the epidermis.Mol Biol Cell 32, no. 21 (November 1, 2021): ar29. https://doi.org/10.1091/mbc.E21-06-0291.
Moreci RS, Lechler T. KIF18B is a cell type-specific regulator of spindle orientation in the epidermis. Mol Biol Cell. 2021 Nov 1;32(21):ar29.
Moreci, Rebecca S., and Terry Lechler. “KIF18B is a cell type-specific regulator of spindle orientation in the epidermis.Mol Biol Cell, vol. 32, no. 21, Nov. 2021, p. ar29. Pubmed, doi:10.1091/mbc.E21-06-0291.
Moreci RS, Lechler T. KIF18B is a cell type-specific regulator of spindle orientation in the epidermis. Mol Biol Cell. 2021 Nov 1;32(21):ar29.

Published In

Mol Biol Cell

DOI

EISSN

1939-4586

Publication Date

November 1, 2021

Volume

32

Issue

21

Start / End Page

ar29

Location

United States

Related Subject Headings

  • Spindle Apparatus
  • Primary Cell Culture
  • Mitosis
  • Microtubules
  • Mice, Knockout
  • Mice
  • Male
  • Kinesins
  • Keratinocytes
  • Female