Convergent evolution of bacterial ceramide synthesis.

Journal Article (Journal Article)

The bacterial domain produces numerous types of sphingolipids with various physiological functions. In the human microbiome, commensal and pathogenic bacteria use these lipids to modulate the host inflammatory system. Despite their growing importance, their biosynthetic pathway remains undefined since several key eukaryotic ceramide synthesis enzymes have no bacterial homolog. Here we used genomic and biochemical approaches to identify six proteins comprising the complete pathway for bacterial ceramide synthesis. Bioinformatic analyses revealed the widespread potential for bacterial ceramide synthesis leading to our discovery of a Gram-positive species that produces ceramides. Biochemical evidence demonstrated that the bacterial pathway operates in a different order from that in eukaryotes. Furthermore, phylogenetic analyses support the hypothesis that the bacterial and eukaryotic ceramide pathways evolved independently.

Full Text

Duke Authors

Cited Authors

  • Stankeviciute, G; Tang, P; Ashley, B; Chamberlain, JD; Hansen, MEB; Coleman, A; D'Emilia, R; Fu, L; Mohan, EC; Nguyen, H; Guan, Z; Campopiano, DJ; Klein, EA

Published Date

  • March 2022

Published In

Volume / Issue

  • 18 / 3

Start / End Page

  • 305 - 312

PubMed ID

  • 34969973

Pubmed Central ID

  • PMC8891067

Electronic International Standard Serial Number (EISSN)

  • 1552-4469

Digital Object Identifier (DOI)

  • 10.1038/s41589-021-00948-7


  • eng

Conference Location

  • United States