In Vitro Differentiation of Tumor-Associated Macrophages from Monocyte Precursors with Modified Melanoma-Conditioned Medium.
Tumor-associated macrophages (TAMs) are one of most important components of the tumor microenvironment. Although many assays have been developed to differentiate monocytes into macrophages (Mϕ) for studying the biology of TAMs in vitro, little is known whether the macrophages induced by these approaches can recapitulate the biology of TAMs present in the tumor microenvironment. We have developed a novel assay to differentiate human monocytes into TAMs using modified melanoma-conditioned medium, which is derived from the concentrated tumor cell culture medium. Characterization of these modified melanoma-conditioned medium-induced macrophages (MCMI-Mϕ) by multiple flow cytometry, Luminex, microarray, and immunohistochemistry analyses indicates that MCMI-Mϕ are phenotypically and functionally highly similar to the TAMs present in the tumor microenvironment.
Duke Scholars
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Related Subject Headings
- Tumor-Associated Macrophages
- Tumor Microenvironment
- Monocytes
- Melanoma
- Macrophages
- Leukocytes, Mononuclear
- Humans
- Developmental Biology
- Culture Media, Conditioned
- Cell Line, Tumor
Citation
DOI
Publication Date
Volume
Start / End Page
Related Subject Headings
- Tumor-Associated Macrophages
- Tumor Microenvironment
- Monocytes
- Melanoma
- Macrophages
- Leukocytes, Mononuclear
- Humans
- Developmental Biology
- Culture Media, Conditioned
- Cell Line, Tumor