Hemoglobin switching in sheep. Cloning and characterization of the beta A and beta-like embryonic globin genes from genomic DNA.

Journal Article (Journal Article)

Genomic DNA from a fetal sheep homozygous for the beta A gene was used to construct a library of one million cloned DNA fragments using the bacteriophage vector, Charon 4A. Screening of 150,000 plaques from this library using radioactive beta-globin gene sequences resulted in the isolation of two recombinant bacteriophage containing globin genes. One of these, S beta AG-21, contains the complete adult beta A-globin gene as demonstrated by hybridization and restriction endonuclease analysis. In common with adult globin genes from other species, the beta A gene contains small (105 base pairs) and large (900 base pairs) intervening sequences. The second recombinant bacteriophage, SG-4, contains a complete embryonic beta-like globin gene which is expressed in the sheep embryo as demonstrated by hybridization analysis with cDNA made from sheep embryonic globin mRNA. Although differing in its restriction endonuclease map from the adult beta-globin genes, SG-4 appears to contain a large intervening sequence of at least 750 base pairs in length. Finally, preliminary evidence is discussed which indicates that a Pvu II site just 5' to the Cap site may be a common feature of sheep globin genes.

Full Text

Duke Authors

Cited Authors

  • Kretschmer, PJ; Kaufman, RE; Coon, HC; Chen, MJ; Geist, CE; Nienhuis, AW

Published Date

  • April 10, 1980

Published In

Volume / Issue

  • 255 / 7

Start / End Page

  • 3204 - 3211

PubMed ID

  • 6244312

International Standard Serial Number (ISSN)

  • 0021-9258


  • eng

Conference Location

  • United States