Cloning and characterization of DNA sequences surrounding the human gamma-, delta-, and beta-globin genes.

Journal Article (Journal Article)

The human gamma-, delta-, and beta-globin genes are located within a 30-kilobase (kb) region of DNA, of which only 20% represents the globin genes. We have attempted to define the nature of flanking and intergenic sequences by isolating recombinants containing the human epsilon, both gamma-, or the 3' end of the beta-globin gene from a bacteriophage library of cloned human DNA. Comparison of these recombinants and a recombinant containing the delta- and beta-globin genes (H beta G1) has provided the following results. The epsilon-globin gene is located 14 kb 5' to the G gamma gene. DNA sequence homology between the region containing the two G gamma genes and the delta nd beta gene region is limited to only a few hundred nucleotides which include the globin coding sequences. Repetitive DNA sequences have been found in the region 3' to the beta-globin gene. Sequences located adjacent to the beta-globin gene are repeated in the globin gene region. A repetitive DNA sequence more than 3.2 kb long is repeated frequently in the human genome but is not repeated in the globin gene region in the clones examined.

Full Text

Duke Authors

Cited Authors

  • Kaufman, RE; Kretschmer, PJ; Adams, JW; Coon, HC; Anderson, WF; Nienhuis, AW

Published Date

  • July 1, 1980

Published In

Volume / Issue

  • 77 / 7

Start / End Page

  • 4229 - 4233

PubMed ID

  • 6254016

Pubmed Central ID

  • PMC349805

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.77.7.4229


  • eng

Conference Location

  • United States