Skin Injury Activates a Rapid TRPV1-Dependent Antiviral Protein Response.

Journal Article (Journal Article)

The skin serves as the interface between the body and the environment and plays a fundamental role in innate antimicrobial host immunity. Antiviral proteins (AVPs) are part of the innate host defense system and provide protection against viral pathogens. How breach of the skin barrier influences innate AVP production remains largely unknown. In this study, we characterized the induction and regulation of AVPs after skin injury and identified a key role of TRPV1 in this process. Transcriptional and phenotypic profiling of cutaneous wounds revealed that skin injury induces high levels of AVPs in both mice and humans. Remarkably, pharmacologic and genetic ablation of TRPV1-mediated nociception abrogated the induction of AVPs, including Oas2, Oasl2, and Isg15 after skin injury in mice. Conversely, stimulation of TRPV1 nociceptors was sufficient to induce AVP production involving the CD301b+ cells‒IL-27‒mediated signaling pathway. Using IL-27 receptor‒knockout mice, we show that IL-27 signaling is required in the induction of AVPs after skin injury. Finally, loss of TRPV1 signaling leads to increased viral infectivity of herpes simplex virus. Together, our data indicate that TRPV1 signaling ensures skin antiviral competence on wounding.

Full Text

Duke Authors

Cited Authors

  • Lei, V; Handfield, C; Kwock, JT; Kirchner, SJ; Lee, MJ; Coates, M; Wang, K; Han, Q; Wang, Z; Powers, JG; Wolfe, S; Corcoran, DL; Fanelli, B; Dadlani, M; Ji, R-R; Zhang, JY; MacLeod, AS

Published Date

  • August 2022

Published In

Volume / Issue

  • 142 / 8

Start / End Page

  • 2249 - 2259.e9

PubMed ID

  • 35007556

Pubmed Central ID

  • PMC9259761

Electronic International Standard Serial Number (EISSN)

  • 1523-1747

Digital Object Identifier (DOI)

  • 10.1016/j.jid.2021.11.041

Language

  • eng

Conference Location

  • United States