Evaluating cytokine production by flow cytometry using brefeldin A in mice.

Journal Article (Journal Article)

Characterizing cytokine production in situ is important for properly understanding immunologic responses. Cytokine reporter mice are limited by the need to cross markers into various knockout backgrounds and by availability of reporters of interest. To overcome this, we utilize injection of brefeldin A into mice to enable flow cytometric analysis of in situ cytokine production during a bacterial infection. While we evaluate IFN-γ production during Burkholderia thailandensis infection, this protocol can be applied to other cytokines and other mouse models. For complete details on the use and execution of this protocol, please refer to Kovacs et al. (2020) and Liu and Whitton (2005).

Full Text

Duke Authors

Cited Authors

  • Kovacs, SB; Oh, C; Aachoui, Y; Miao, EA

Published Date

  • March 19, 2021

Published In

Volume / Issue

  • 2 / 1

Start / End Page

  • 100244 -

PubMed ID

  • 33458706

Pubmed Central ID

  • PMC7797915

Electronic International Standard Serial Number (EISSN)

  • 2666-1667

Digital Object Identifier (DOI)

  • 10.1016/j.xpro.2020.100244


  • eng

Conference Location

  • United States