Assessing locomotor activity in larval zebrafish: Influence of extrinsic and intrinsic variables.

Journal Article (Journal Article)

The U.S. Environmental Protection Agency is evaluating methods to screen and prioritize large numbers of chemicals for developmental toxicity. We are exploring methods to detect developmentally neurotoxic chemicals using zebrafish behavior at 6 days of age. The behavioral paradigm simultaneously tests individual larval zebrafish under both light and dark conditions in a 96-well plate using a video tracking system. We have found that many variables affect the level or pattern of locomotor activity, including age of the larvae, size of the well, and the presence of malformations. Some other variables, however, do not appear to affect larval behavior including type of rearing solution (10% Hank's vs. 1:3 Danieau vs 60 mg/kg Instant Ocean vs 1× and 1:10× EPA Moderately Hard Water). Zebrafish larval behavior using a microtiter plate format may be an ideal endpoint for screening developmentally neurotoxic chemicals, but it is imperative that many test variables be carefully specified and controlled.

Full Text

Duke Authors

Cited Authors

  • Padilla, S; Hunter, DL; Padnos, B; Frady, S; MacPhail, RC

Published Date

  • November 2011

Published In

Volume / Issue

  • 33 / 6

Start / End Page

  • 624 - 630

PubMed ID

  • 21871562

Electronic International Standard Serial Number (EISSN)

  • 1872-9738

International Standard Serial Number (ISSN)

  • 0892-0362

Digital Object Identifier (DOI)

  • 10.1016/


  • eng