Skip to main content
Journal cover image

Adverse outcome pathways during early fish development: a conceptual framework for identification of chemical screening and prioritization strategies.

Publication ,  Journal Article
Volz, DC; Belanger, S; Embry, M; Padilla, S; Sanderson, H; Schirmer, K; Scholz, S; Villeneuve, D
Published in: Toxicological sciences : an official journal of the Society of Toxicology
October 2011

The fish early life-stage (FELS) test guideline (OECD 210 or OCSPP 850.1400) is the most frequently used bioassay for predicting chronic fish toxicity and supporting aquatic ecological risk assessments around the world. For each chemical, the FELS test requires a minimum of 360 fish and 1 to 3 months from test initiation to termination. Although valuable for predicting fish full life-cycle toxicity, FELS tests are labor and resource intensive and, due to an emphasis on apical endpoints, provide little to no information about chemical mode of action. Therefore, the development and implementation of alternative testing strategies for screening and prioritizing chemicals has the potential to reduce the cost and number of animals required for estimating FELS toxicity and, at the same time, provides insights into mechanisms of toxicity. Using three reference chemicals with well-established yet distinct adverse outcome pathways (AOPs) in early life stages of fish, we proposed FELS-specific AOPs as conceptual frameworks for identifying useful chemical screening and prioritization strategies. The reference chemicals selected as case studies were a cardiotoxic aryl hydrocarbon receptor agonist (2,3,7,8-tetrachlorodibenzo-p-dioxin), neurotoxic acetylcholinesterase inhibitor (chlorpyrifos), and narcotic surfactant (linear alkylbenzene sulfonate). Using qualitative descriptions for each chemical during early fish development, we developed generalized AOPs and, based on these examples, proposed a three-tiered testing strategy for screening and prioritizing chemicals for FELS testing. Linked with biologically based concentration-response models, a tiered testing strategy may help reduce the reliance on long-term and costly FELS tests required for assessing the hazard of thousands of chemicals currently in commerce.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Toxicological sciences : an official journal of the Society of Toxicology

DOI

EISSN

1096-0929

ISSN

1096-6080

Publication Date

October 2011

Volume

123

Issue

2

Start / End Page

349 / 358

Related Subject Headings

  • Toxicology
  • Toxicity Tests, Chronic
  • Risk Assessment
  • Research Design
  • Polychlorinated Dibenzodioxins
  • Mass Screening
  • Life Cycle Stages
  • High-Throughput Screening Assays
  • Health Priorities
  • Hazardous Substances
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Volz, D. C., Belanger, S., Embry, M., Padilla, S., Sanderson, H., Schirmer, K., … Villeneuve, D. (2011). Adverse outcome pathways during early fish development: a conceptual framework for identification of chemical screening and prioritization strategies. Toxicological Sciences : An Official Journal of the Society of Toxicology, 123(2), 349–358. https://doi.org/10.1093/toxsci/kfr185
Volz, David C., Scott Belanger, Michelle Embry, Stephanie Padilla, Hans Sanderson, Kristin Schirmer, Stefan Scholz, and Daniel Villeneuve. “Adverse outcome pathways during early fish development: a conceptual framework for identification of chemical screening and prioritization strategies.Toxicological Sciences : An Official Journal of the Society of Toxicology 123, no. 2 (October 2011): 349–58. https://doi.org/10.1093/toxsci/kfr185.
Volz DC, Belanger S, Embry M, Padilla S, Sanderson H, Schirmer K, et al. Adverse outcome pathways during early fish development: a conceptual framework for identification of chemical screening and prioritization strategies. Toxicological sciences : an official journal of the Society of Toxicology. 2011 Oct;123(2):349–58.
Volz, David C., et al. “Adverse outcome pathways during early fish development: a conceptual framework for identification of chemical screening and prioritization strategies.Toxicological Sciences : An Official Journal of the Society of Toxicology, vol. 123, no. 2, Oct. 2011, pp. 349–58. Epmc, doi:10.1093/toxsci/kfr185.
Volz DC, Belanger S, Embry M, Padilla S, Sanderson H, Schirmer K, Scholz S, Villeneuve D. Adverse outcome pathways during early fish development: a conceptual framework for identification of chemical screening and prioritization strategies. Toxicological sciences : an official journal of the Society of Toxicology. 2011 Oct;123(2):349–358.
Journal cover image

Published In

Toxicological sciences : an official journal of the Society of Toxicology

DOI

EISSN

1096-0929

ISSN

1096-6080

Publication Date

October 2011

Volume

123

Issue

2

Start / End Page

349 / 358

Related Subject Headings

  • Toxicology
  • Toxicity Tests, Chronic
  • Risk Assessment
  • Research Design
  • Polychlorinated Dibenzodioxins
  • Mass Screening
  • Life Cycle Stages
  • High-Throughput Screening Assays
  • Health Priorities
  • Hazardous Substances