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Modulation of neurotoxic esterase activity in vitro by phospholipids.

Publication ,  Journal Article
Pope, CN; Padilla, S
Published in: Toxicology and applied pharmacology
February 1989

Neurotoxic esterase (NTE), the proposed molecular site for the initiation of organophosphorus-induced delayed neuropathy, is a membrane-associated enzyme. NTE activity was solubilized from chicken brain microsomal membranes with the detergent 3-[(3-cholamidopropyl)dimethylammonio]-1- propanesulfonate and partially separated from other solubilized hen brain esterases by DEAE-Sephacel anion-exchange chromatography using stepwise increases in salt concentration; however, there was poor recovery of NTE activity and only a slight increase in NTE specific activity. NTE activity in the "high salt" fraction (i.e., the NTE-enriched fraction) was markedly activated by a heat-stable factor(s) present in other fractions eluted from the column. This activating factor was extracted with organic solvents, suggesting that it may be lipid. In a related study, purified phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine were also found to activate the partially separated NTE activity in a concentration-dependent manner while phosphatidyl-inositol was found to inhibit the same partially separated NTE fraction in a concentration-dependent manner. The results suggest that lipids may modulate NTE activity and that the loss of lipid cofactors during chromatographic separations may underlie some of the difficulties encountered in isolation of active NTE.

Duke Scholars

Published In

Toxicology and applied pharmacology

DOI

EISSN

1096-0333

ISSN

0041-008X

Publication Date

February 1989

Volume

97

Issue

2

Start / End Page

272 / 278

Related Subject Headings

  • Toxicology
  • Phospholipids
  • Hot Temperature
  • Female
  • Dose-Response Relationship, Drug
  • Chromatography, Ion Exchange
  • Chickens
  • Carboxylic Ester Hydrolases
  • Animals
  • 3214 Pharmacology and pharmaceutical sciences
 

Citation

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Pope, C. N., & Padilla, S. (1989). Modulation of neurotoxic esterase activity in vitro by phospholipids. Toxicology and Applied Pharmacology, 97(2), 272–278. https://doi.org/10.1016/0041-008x(89)90332-3
Pope, C. N., and S. Padilla. “Modulation of neurotoxic esterase activity in vitro by phospholipids.Toxicology and Applied Pharmacology 97, no. 2 (February 1989): 272–78. https://doi.org/10.1016/0041-008x(89)90332-3.
Pope CN, Padilla S. Modulation of neurotoxic esterase activity in vitro by phospholipids. Toxicology and applied pharmacology. 1989 Feb;97(2):272–8.
Pope, C. N., and S. Padilla. “Modulation of neurotoxic esterase activity in vitro by phospholipids.Toxicology and Applied Pharmacology, vol. 97, no. 2, Feb. 1989, pp. 272–78. Epmc, doi:10.1016/0041-008x(89)90332-3.
Pope CN, Padilla S. Modulation of neurotoxic esterase activity in vitro by phospholipids. Toxicology and applied pharmacology. 1989 Feb;97(2):272–278.
Journal cover image

Published In

Toxicology and applied pharmacology

DOI

EISSN

1096-0333

ISSN

0041-008X

Publication Date

February 1989

Volume

97

Issue

2

Start / End Page

272 / 278

Related Subject Headings

  • Toxicology
  • Phospholipids
  • Hot Temperature
  • Female
  • Dose-Response Relationship, Drug
  • Chromatography, Ion Exchange
  • Chickens
  • Carboxylic Ester Hydrolases
  • Animals
  • 3214 Pharmacology and pharmaceutical sciences