Determination of acrylamide in rat serum and sciatic nerve by gas chromatography-electron-capture detection.

Journal Article (Journal Article)

A modified method for the derivatization and determination of acrylamide as 2-bromopropenamide by gas chromatography-electron-capture detection was developed and applied to serum and sciatic nerve from rats. The method was accurate and precise over the calibration range 2.24-7.47 micrograms/ml in serum diluted 1:125 and 4-122 micrograms/g in sciatic nerve homogenate (5 mg/ml). limits of detection were estimated to be 1200 ng/ml in undiluted serum and 3 micrograms/g in intact sciatic nerve. The use of less dilute samples to allow for lower limits of detection appears feasible. The time-course of acrylamide in serum and sciatic nerve was studied after acute dosing and indicated elimination half-lives of 1.8 and 2.0 h for serum and sciatic nerve, respectively. A dose-effect relationship was established for each matrix after acute dosing and the measured acrylamide concentrations in serum (microgram/ml) were approximately the same as in sciatic nerve (microgram/g).

Full Text

Duke Authors

Cited Authors

  • Raymer, JH; Sparacino, CM; Velez, GR; Padilla, S; MacPhail, RC; Crofton, KM

Published Date

  • September 1993

Published In

  • Journal of Chromatography

Volume / Issue

  • 619 / 2

Start / End Page

  • 223 - 234

PubMed ID

  • 8263094

Digital Object Identifier (DOI)

  • 10.1016/0378-4347(93)80111-g


  • eng