Factors in standardizing automated cholinesterase assays.

Journal Article (Multicenter Study;Journal Article)

A scientific panel assembled by the U.S. Environmental Protection Agency (EPA) determined that variability in cholinesterase (ChE) activities in the agency's pesticide/animal study database likely was due to a lack of accepted guidelines for ChE methodology. A series of trials was held in which participating laboratories measured ChE activity in blood and brain samples from untreated and pesticide-treated rats using a colorimetric assay method. The degree of inhibition of ChE activity in plasma and brain samples compared to controls was consistent among most of the laboratories. The ChE activity in erythrocyte samples differed more between laboratories due to a high blank, low erythrocyte AChE activity and hemoglobin absorption at the wavelength of the assay. Strategies are suggested for minimizing the variability of ChE activity in hemoglobin-rich samples.

Full Text

Duke Authors

Cited Authors

  • Wilson, BW; Padilla, S; Henderson, JD; Brimijoin, S; Dass, PD; Elliot, G; Jaeger, B; Lanz, D; Pearson, R; Spies, R

Published Date

  • June 1996

Published In

Volume / Issue

  • 48 / 2

Start / End Page

  • 187 - 195

PubMed ID

  • 8642625

International Standard Serial Number (ISSN)

  • 0098-4108

Digital Object Identifier (DOI)

  • 10.1080/009841096161429


  • eng