Loss of adult skeletal muscle stem cells drives age-related neuromuscular junction degeneration.

Journal Article (Journal Article)

Neuromuscular junction degeneration is a prominent aspect of sarcopenia, the age-associated loss of skeletal muscle integrity. Previously, we showed that muscle stem cells activate and contribute to mouse neuromuscular junction regeneration in response to denervation (Liu et al., 2015). Here, we examined gene expression profiles and neuromuscular junction integrity in aged mouse muscles, and unexpectedly found limited denervation despite a high level of degenerated neuromuscular junctions. Instead, degenerated neuromuscular junctions were associated with reduced contribution from muscle stem cells. Indeed, muscle stem cell depletion was sufficient to induce neuromuscular junction degeneration at a younger age. Conversely, prevention of muscle stem cell and derived myonuclei loss was associated with attenuation of age-related neuromuscular junction degeneration, muscle atrophy, and the promotion of aged muscle force generation. Our observations demonstrate that deficiencies in muscle stem cell fate and post-synaptic myogenesis provide a cellular basis for age-related neuromuscular junction degeneration and associated skeletal muscle decline.

Full Text

Duke Authors

Cited Authors

  • Liu, W; Klose, A; Forman, S; Paris, ND; Wei-LaPierre, L; Cortés-Lopéz, M; Tan, A; Flaherty, M; Miura, P; Dirksen, RT; Chakkalakal, JV

Published Date

  • June 6, 2017

Published In

Volume / Issue

  • 6 /

PubMed ID

  • 28583253

Pubmed Central ID

  • PMC5462534

Electronic International Standard Serial Number (EISSN)

  • 2050-084X

Digital Object Identifier (DOI)

  • 10.7554/eLife.26464

Language

  • eng

Conference Location

  • England