Skip to main content

Two glutathione peroxidases in the fungal pathogen Cryptococcus neoformans are expressed in the presence of specific substrates.

Publication ,  Journal Article
Missall, TA; Cherry-Harris, JF; Lodge, JK
Published in: Microbiology (Reading)
August 2005

Glutathione peroxidases catalyse the reduction of peroxides by reduced glutathione. To determine if these enzymes are important for resistance to oxidative stress and evasion of the innate immune system by the fungal pathogen Cryptococcus neoformans, two glutathione peroxidase homologues, which share 38 % identity, were identified and investigated. In this study, these peroxidases, Gpx1 and Gpx2, their localization, their contribution to total glutathione peroxidase activity, and their importance to the oxidative and nitrosative stress resistance of C. neoformans are described. It is shown that the two glutathione peroxidase genes are differentially expressed in response to stress. While both GPX1 and GPX2 are induced during t-butylhydroperoxide or cumene hydroperoxide stress and repressed during nitric oxide stress, only GPX2 is induced in response to hydrogen peroxide stress. Deletion mutants of each and both of the glutathione peroxidases were generated, and it was found that they are sensitive to various peroxide stresses while showing wild-type resistance to other oxidant stresses, such as superoxide and nitric oxide. While the glutathione peroxidase mutants are slightly sensitive to oxidant killing by macrophages, they exhibit wild-type virulence in a mouse model of cryptococcosis.

Duke Scholars

Published In

Microbiology (Reading)

DOI

ISSN

1350-0872

Publication Date

August 2005

Volume

151

Issue

Pt 8

Start / End Page

2573 / 2581

Location

England

Related Subject Headings

  • Substrate Specificity
  • Oxidative Stress
  • Microbiology
  • Mice
  • Glutathione Peroxidase
  • Gene Expression Regulation, Fungal
  • Cryptococcus neoformans
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Missall, T. A., Cherry-Harris, J. F., & Lodge, J. K. (2005). Two glutathione peroxidases in the fungal pathogen Cryptococcus neoformans are expressed in the presence of specific substrates. Microbiology (Reading), 151(Pt 8), 2573–2581. https://doi.org/10.1099/mic.0.28132-0
Missall, Tricia A., Jocie F. Cherry-Harris, and Jennifer K. Lodge. “Two glutathione peroxidases in the fungal pathogen Cryptococcus neoformans are expressed in the presence of specific substrates.Microbiology (Reading) 151, no. Pt 8 (August 2005): 2573–81. https://doi.org/10.1099/mic.0.28132-0.
Missall TA, Cherry-Harris JF, Lodge JK. Two glutathione peroxidases in the fungal pathogen Cryptococcus neoformans are expressed in the presence of specific substrates. Microbiology (Reading). 2005 Aug;151(Pt 8):2573–81.
Missall, Tricia A., et al. “Two glutathione peroxidases in the fungal pathogen Cryptococcus neoformans are expressed in the presence of specific substrates.Microbiology (Reading), vol. 151, no. Pt 8, Aug. 2005, pp. 2573–81. Pubmed, doi:10.1099/mic.0.28132-0.
Missall TA, Cherry-Harris JF, Lodge JK. Two glutathione peroxidases in the fungal pathogen Cryptococcus neoformans are expressed in the presence of specific substrates. Microbiology (Reading). 2005 Aug;151(Pt 8):2573–2581.

Published In

Microbiology (Reading)

DOI

ISSN

1350-0872

Publication Date

August 2005

Volume

151

Issue

Pt 8

Start / End Page

2573 / 2581

Location

England

Related Subject Headings

  • Substrate Specificity
  • Oxidative Stress
  • Microbiology
  • Mice
  • Glutathione Peroxidase
  • Gene Expression Regulation, Fungal
  • Cryptococcus neoformans
  • Animals