Expression of polyoma early gene products in E. coli.

Journal Article (Journal Article)

The three products of the early region of polyoma virus have been cloned for expression in E. coli using the Tac promoter. Although the identical promoter and ribosome binding site are used in each final construction, the observed level of protein expression is different for each protein. While plasmids expressing wild type T antigens as well as a plasmid expressing the truncated Py-1387T middle T antigen lacking the membrane-anchoring sequence give rise to synthesis of proteins readily detectible by 35S-methionine labeling and immunoprecipitation, only small T and the middle T of Py-1387T are made in amounts sufficient for ready detection in total cell protein. Unlike middle T expressed in animal cells, middle T produced in E. coli is not detectibly phosphorylated. Further, the E. coli protein lacks tyrosine kinase activity.

Full Text

Duke Authors

Cited Authors

  • Schaffhausen, B; Benjamin, TL; Lodge, J; Kaplan, D; Roberts, TM

Published Date

  • January 25, 1985

Published In

Volume / Issue

  • 13 / 2

Start / End Page

  • 501 - 519

PubMed ID

  • 2987799

Pubmed Central ID

  • PMC341011

International Standard Serial Number (ISSN)

  • 0305-1048

Digital Object Identifier (DOI)

  • 10.1093/nar/13.2.501

Language

  • eng

Conference Location

  • England