Broad-spectrum virus resistance in transgenic plants expressing pokeweed antiviral protein.

Journal Article (Journal Article)

Exogenous application of pokeweed antiviral protein (PAP), a ribosome-inhibiting protein found in the cell walls of Phytolacca americana (pokeweed), protects heterologous plants from viral infection. A cDNA clone for PAP was isolated and introduced into tobacco and potato plants by transformation with Agrobacterium tumefaciens. Transgenic plants that expressed either PAP or a double mutant derivative of PAP showed resistance to infection by different viruses. Resistance was effective against both mechanical and aphid transmission. Analysis of the vacuum infiltrate of leaves expressing PAP showed that it is enriched in the intercellular fluid. Analysis of resistance in transgenic plants suggests that PAP confers viral resistance by inhibiting an early event in infection. Previous methods for creating virus-resistant plants have been specific for a particular virus or closely related viruses. To protect plants against more than one virus, multiple genes must be introduced and expressed in a single transgenic line. Expression of PAP in transgenic plants offers the possibility of developing resistance to a broad spectrum of plant viruses by expression of a single gene.

Full Text

Duke Authors

Cited Authors

  • Lodge, JK; Kaniewski, WK; Tumer, NE

Published Date

  • August 1, 1993

Published In

Volume / Issue

  • 90 / 15

Start / End Page

  • 7089 - 7093

PubMed ID

  • 8346221

Pubmed Central ID

  • PMC47081

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.90.15.7089


  • eng

Conference Location

  • United States