Assessment of Intracellular GTP Levels Using Genetically Encoded Fluorescent Sensors.
Publication
, Chapter
Bianchi-Smiraglia, A; Nikiforov, MA
January 2022
Changes in intracellular GTP levels, even incremental ones, profoundly affect the activity of several GTP-binding proteins ultimately resulting in alteration of several basal cellular phenotypes including cell motility, invasion, and tumorigenesis. However, until recently, no tools were available for GTP quantification in live cells. Therefore, in the current chapter, we describe the methodology for the quantitative assessment of spatiotemporal changes in GTP levels in the cells using genetically encoded fluorescent ratiometric GTP sensors termed GEVALs for GTP evaluators.
DOI
Publication Date
January 2022
Volume
2394
Start / End Page
163 / 169
Related Subject Headings
- rhoA GTP-Binding Protein
- Guanosine Triphosphate
- Fluorescent Dyes
- Developmental Biology
- Cell Movement
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Bianchi-Smiraglia, A., & Nikiforov, M. A. (2022). Assessment of Intracellular GTP Levels Using Genetically Encoded Fluorescent Sensors. (Vol. 2394, pp. 163–169). https://doi.org/10.1007/978-1-0716-1811-0_10
Bianchi-Smiraglia, Anna, and Mikhail A. Nikiforov. “Assessment of Intracellular GTP Levels Using Genetically Encoded Fluorescent Sensors.,” 2394:163–69, 2022. https://doi.org/10.1007/978-1-0716-1811-0_10.
Bianchi-Smiraglia A, Nikiforov MA. Assessment of Intracellular GTP Levels Using Genetically Encoded Fluorescent Sensors. In 2022. p. 163–9.
Bianchi-Smiraglia, Anna, and Mikhail A. Nikiforov. Assessment of Intracellular GTP Levels Using Genetically Encoded Fluorescent Sensors. Vol. 2394, 2022, pp. 163–69. Epmc, doi:10.1007/978-1-0716-1811-0_10.
Bianchi-Smiraglia A, Nikiforov MA. Assessment of Intracellular GTP Levels Using Genetically Encoded Fluorescent Sensors. 2022. p. 163–169.
DOI
Publication Date
January 2022
Volume
2394
Start / End Page
163 / 169
Related Subject Headings
- rhoA GTP-Binding Protein
- Guanosine Triphosphate
- Fluorescent Dyes
- Developmental Biology
- Cell Movement
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences