Assessment of Intracellular GTP Levels Using Genetically Encoded Fluorescent Sensors.
Book Section
Changes in intracellular GTP levels, even incremental ones, profoundly affect the activity of several GTP-binding proteins ultimately resulting in alteration of several basal cellular phenotypes including cell motility, invasion, and tumorigenesis. However, until recently, no tools were available for GTP quantification in live cells. Therefore, in the current chapter, we describe the methodology for the quantitative assessment of spatiotemporal changes in GTP levels in the cells using genetically encoded fluorescent ratiometric GTP sensors termed GEVALs for GTP evaluators.
Full Text
Duke Authors
Cited Authors
- Bianchi-Smiraglia, A; Nikiforov, MA
Published Date
- 2022
Volume / Issue
- 2394 /
Start / End Page
- 163 - 169
PubMed ID
- 35094327
Digital Object Identifier (DOI)
- 10.1007/978-1-0716-1811-0_10