Quantitative Jones matrix imaging using vectorial Fourier ptychography.

Journal Article (Journal Article)

This paper presents a microscopic imaging technique that uses variable-angle illumination to recover the complex polarimetric properties of a specimen at high resolution and over a large field-of-view. The approach extends Fourier ptychography, which is a synthetic aperture-based imaging approach to improve resolution with phaseless measurements, to additionally account for the vectorial nature of light. After images are acquired using a standard microscope outfitted with an LED illumination array and two polarizers, our vectorial Fourier ptychography (vFP) algorithm solves for the complex 2x2 Jones matrix of the anisotropic specimen of interest at each resolved spatial location. We introduce a new sequential Gauss-Newton-based solver that additionally jointly estimates and removes polarization-dependent imaging system aberrations. We demonstrate effective vFP performance by generating large-area (29 mm2), high-resolution (1.24 μm full-pitch) reconstructions of sample absorption, phase, orientation, diattenuation, and retardance for a variety of calibration samples and biological specimens.

Full Text

Duke Authors

Cited Authors

  • Dai, X; Xu, S; Yang, X; Zhou, KC; Glass, C; Konda, PC; Horstmeyer, R

Published Date

  • March 1, 2022

Published In

Volume / Issue

  • 13 / 3

Start / End Page

  • 1457 - 1470

PubMed ID

  • 35414998

Pubmed Central ID

  • PMC8973192

International Standard Serial Number (ISSN)

  • 2156-7085

Digital Object Identifier (DOI)

  • 10.1364/BOE.448804


  • eng

Conference Location

  • United States