Mutational profiling of therapy-related myelodysplastic syndromes and acute myeloid leukemia by next generation sequencing, a comparison with de novo diseases.

Journal Article (Journal Article)

In this study we used a next generation sequencing-based approach to profile gene mutations in therapy-related myelodysplastic syndromes (t-MDS) and acute myeloid leukemia (t-AML); and compared these findings with de novo MDS/AML. Consecutive bone marrow samples of 498 patients, including 70 therapy-related (28 MDS and 42 AML) and 428 de novo (147 MDS and 281 AML) were analyzed using a modified-TruSeq Amplicon Cancer Panel (Illumina) covering mutation hotspots of 53 genes. Overall, mutation(s) were detected in 58.6% of t-MDS/AML and 56.8% of de novo MDS/AML. Of therapy-related cases, mutations were detected in 71.4% of t-AML versus 39.3% t-MDS (p=0.0127). TP53 was the most common mutated gene in t-MDS (35.7%) as well as t-AML (33.3%), significantly higher than de novo MDS (17.7%) (p=0.0410) and de novo AML (12.8%) (p=0.0020). t-AML showed more frequent PTPN11 but less NPM1 and FLT3 mutations than de novo AML. In summary, t-MDS/AML shows a mutation profile different from their de novo counterparts. TP53 mutations are highly and similarly prevalent in t-MDS and t-AML but mutations in genes other than TP53 were more frequent in t-AML than t-MDS. The molecular genetic profiling further expands our understanding in this group of clinically aggressive yet heterogeneous myeloid neoplasms.

Full Text

Duke Authors

Cited Authors

  • Ok, CY; Patel, KP; Garcia-Manero, G; Routbort, MJ; Fu, B; Tang, G; Goswami, M; Singh, R; Kanagal-Shamanna, R; Pierce, SA; Young, KH; Kantarjian, HM; Medeiros, LJ; Luthra, R; Wang, SA

Published Date

  • March 2015

Published In

Volume / Issue

  • 39 / 3

Start / End Page

  • 348 - 354

PubMed ID

  • 25573287

Pubmed Central ID

  • PMC5548131

Electronic International Standard Serial Number (EISSN)

  • 1873-5835

Digital Object Identifier (DOI)

  • 10.1016/j.leukres.2014.12.006

Language

  • eng

Conference Location

  • England