An In Vitro Microfluidic Alveolus Model to Study Lung Biomechanics.

Journal Article (Journal Article)

The gas exchange units of the lung, the alveoli, are mechanically active and undergo cyclic deformation during breathing. The epithelial cells that line the alveoli contribute to lung function by reducing surface tension via surfactant secretion, which is highly influenced by the breathing-associated mechanical cues. These spatially heterogeneous mechanical cues have been linked to several physiological and pathophysiological states. Here, we describe the development of a microfluidically assisted lung cell culture model that incorporates heterogeneous cyclic stretching to mimic alveolar respiratory motions. Employing this device, we have examined the effects of respiratory biomechanics (associated with breathing-like movements) and strain heterogeneity on alveolar epithelial cell functions. Furthermore, we have assessed the potential application of this platform to model altered matrix compliance associated with lung pathogenesis and ventilator-induced lung injury. Lung microphysiological platforms incorporating human cells and dynamic biomechanics could serve as an important tool to delineate the role of alveolar micromechanics in physiological and pathological outcomes in the lung.

Full Text

Duke Authors

Cited Authors

  • Kumar, V; Madhurakkat Perikamana, SK; Tata, A; Hoque, J; Gilpin, A; Tata, PR; Varghese, S

Published Date

  • 2022

Published In

Volume / Issue

  • 10 /

Start / End Page

  • 848699 -

PubMed ID

  • 35252157

Pubmed Central ID

  • PMC8895303

International Standard Serial Number (ISSN)

  • 2296-4185

Digital Object Identifier (DOI)

  • 10.3389/fbioe.2022.848699

Language

  • eng

Conference Location

  • Switzerland