Principles for the design of multicellular engineered living systems.

Journal Article (Journal Article)

Remarkable progress in bioengineering over the past two decades has enabled the formulation of fundamental design principles for a variety of medical and non-medical applications. These advancements have laid the foundation for building multicellular engineered living systems (M-CELS) from biological parts, forming functional modules integrated into living machines. These cognizant design principles for living systems encompass novel genetic circuit manipulation, self-assembly, cell-cell/matrix communication, and artificial tissues/organs enabled through systems biology, bioinformatics, computational biology, genetic engineering, and microfluidics. Here, we introduce design principles and a blueprint for forward production of robust and standardized M-CELS, which may undergo variable reiterations through the classic design-build-test-debug cycle. This Review provides practical and theoretical frameworks to forward-design, control, and optimize novel M-CELS. Potential applications include biopharmaceuticals, bioreactor factories, biofuels, environmental bioremediation, cellular computing, biohybrid digital technology, and experimental investigations into mechanisms of multicellular organisms normally hidden inside the "black box" of living cells.

Full Text

Duke Authors

Cited Authors

  • Aydin, O; Passaro, AP; Raman, R; Spellicy, SE; Weinberg, RP; Kamm, RD; Sample, M; Truskey, GA; Zartman, J; Dar, RD; Palacios, S; Wang, J; Tordoff, J; Montserrat, N; Bashir, R; Saif, MTA; Weiss, R

Published Date

  • March 2, 2022

Published In

Volume / Issue

  • 6 / 1

Start / End Page

  • 010903 -

PubMed ID

  • 35274072

Pubmed Central ID

  • PMC8893975

Electronic International Standard Serial Number (EISSN)

  • 2473-2877

International Standard Serial Number (ISSN)

  • 2473-2877

Digital Object Identifier (DOI)

  • 10.1063/5.0076635


  • eng