Itavastatin and resveratrol increase triosephosphate isomerase protein in a newly identified variant of TPI deficiency.

Journal Article (Journal Article)

Triosephosphate isomerase (TPI) deficiency (TPI Df) is an untreatable glycolytic enzymopathy that results in hemolytic anemia, progressive muscular impairment and irreversible brain damage. Although there is a 'common' mutation (TPIE105D), other pathogenic mutations have been described. We identified patients who were compound heterozygous for a newly described mutation, TPIQ181P, and the common TPIE105D mutation. Intriguingly, these patients lacked neuropathy or cognitive impairment. We then initiated biochemical and structural studies of TPIQ181P. Surprisingly, we found that purified TPIQ181P protein had markedly impaired catalytic properties whereas crystallographic studies demonstrated that the TPIQ181P mutation resulted in a highly disordered catalytic lid. We propose that genetic complementation occurs between the two alleles, one with little activity (TPIQ181P) and one with low stability (TPIE105D). Consistent with this, TPIQ181P/E105D fibroblasts exhibit a significant reduction in the TPI protein. These data suggest that impaired stability, and not catalytic activity, is a better predictor of TPI Df severity. Lastly, we tested two recently discovered chemical modulators of mutant TPI stability, itavastatin and resveratrol, and observed a significant increase in TPI in TPIQ181P/E105D patient cells.

Full Text

Duke Authors

Cited Authors

  • VanDemark, AP; Hrizo, SL; Eicher, SL; Kowalski, J; Myers, TD; Pfeifer, MR; Riley, KN; Koeberl, DD; Palladino, MJ

Published Date

  • May 1, 2022

Published In

Volume / Issue

  • 15 / 5

PubMed ID

  • 35315486

Pubmed Central ID

  • PMC9150114

Electronic International Standard Serial Number (EISSN)

  • 1754-8411

Digital Object Identifier (DOI)

  • 10.1242/dmm.049261

Language

  • eng

Conference Location

  • England