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Cocaine alters Homer1 natural antisense transcript in the nucleus accumbens.

Publication ,  Journal Article
Sartor, GC; Powell, SK; Velmeshev, D; Lin, DY; Magistri, M; Wiedner, HJ; Malvezzi, AM; Andrade, NS; Faghihi, MA; Wahlestedt, C
Published in: Mol Cell Neurosci
December 2017

Natural antisense transcripts (NATs) are an abundant class of long noncoding RNAs that have recently been shown to be key regulators of chromatin dynamics and gene expression in nervous system development and neurological disorders. However, it is currently unclear if NAT-based mechanisms also play a role in drug-induced neuroadaptations. Aberrant regulation of gene expression is one critical factor underlying the long-lasting behavioral abnormalities that characterize substance use disorder, and it is possible that some drug-induced transcriptional responses are mediated, in part, by perturbations in NAT activity. To test this hypothesis, we used an automated algorithm that mines the NCBI AceView transcriptomics database to identify NAT overlapping genes linked to addiction. We found that 22% of the genes examined contain NATs and that expression of Homer1 natural antisense transcript (Homer1-AS) was altered in the nucleus accumbens (NAc) of mice 2h and 10days following repeated cocaine administration. In in vitro studies, depletion of Homer1-AS lead to an increase in the corresponding sense gene expression, indicating a potential regulatory mechanisms of Homer1 expression by its corresponding antisense transcript. Future in vivo studies are needed to definitely determine a role for Homer1-AS in cocaine-induced behavioral and molecular adaptations.

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Published In

Mol Cell Neurosci

DOI

EISSN

1095-9327

Publication Date

December 2017

Volume

85

Start / End Page

183 / 189

Location

United States

Related Subject Headings

  • RNA, Antisense
  • Nucleus Accumbens
  • Neurology & Neurosurgery
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Homer Scaffolding Proteins
  • Gene Expression Regulation
  • Dopamine Uptake Inhibitors
  • Cocaine
 

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Sartor, G. C., Powell, S. K., Velmeshev, D., Lin, D. Y., Magistri, M., Wiedner, H. J., … Wahlestedt, C. (2017). Cocaine alters Homer1 natural antisense transcript in the nucleus accumbens. Mol Cell Neurosci, 85, 183–189. https://doi.org/10.1016/j.mcn.2017.10.003
Sartor, Gregory C., Samuel K. Powell, Dmitry Velmeshev, David Y. Lin, Marco Magistri, Hannah J. Wiedner, Andrea M. Malvezzi, Nadja S. Andrade, Mohammad A. Faghihi, and Claes Wahlestedt. “Cocaine alters Homer1 natural antisense transcript in the nucleus accumbens.Mol Cell Neurosci 85 (December 2017): 183–89. https://doi.org/10.1016/j.mcn.2017.10.003.
Sartor GC, Powell SK, Velmeshev D, Lin DY, Magistri M, Wiedner HJ, et al. Cocaine alters Homer1 natural antisense transcript in the nucleus accumbens. Mol Cell Neurosci. 2017 Dec;85:183–9.
Sartor, Gregory C., et al. “Cocaine alters Homer1 natural antisense transcript in the nucleus accumbens.Mol Cell Neurosci, vol. 85, Dec. 2017, pp. 183–89. Pubmed, doi:10.1016/j.mcn.2017.10.003.
Sartor GC, Powell SK, Velmeshev D, Lin DY, Magistri M, Wiedner HJ, Malvezzi AM, Andrade NS, Faghihi MA, Wahlestedt C. Cocaine alters Homer1 natural antisense transcript in the nucleus accumbens. Mol Cell Neurosci. 2017 Dec;85:183–189.
Journal cover image

Published In

Mol Cell Neurosci

DOI

EISSN

1095-9327

Publication Date

December 2017

Volume

85

Start / End Page

183 / 189

Location

United States

Related Subject Headings

  • RNA, Antisense
  • Nucleus Accumbens
  • Neurology & Neurosurgery
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Homer Scaffolding Proteins
  • Gene Expression Regulation
  • Dopamine Uptake Inhibitors
  • Cocaine