Spatiotemporal gene expression trajectories reveal developmental hierarchies of the human cortex.

Journal Article (Journal Article)

Systematic analyses of spatiotemporal gene expression trajectories during organogenesis have been challenging because diverse cell types at different stages of maturation and differentiation coexist in the emerging tissues. We identified discrete cell types as well as temporally and spatially restricted trajectories of radial glia maturation and neurogenesis in developing human telencephalon. These lineage-specific trajectories reveal the expression of neurogenic transcription factors in early radial glia and enriched activation of mammalian target of rapamycin signaling in outer radial glia. Across cortical areas, modest transcriptional differences among radial glia cascade into robust typological distinctions among maturing neurons. Together, our results support a mixed model of topographical, typological, and temporal hierarchies governing cell-type diversity in the developing human telencephalon, including distinct excitatory lineages emerging in rostral and caudal cerebral cortex.

Full Text

Duke Authors

Cited Authors

  • Nowakowski, TJ; Bhaduri, A; Pollen, AA; Alvarado, B; Mostajo-Radji, MA; Di Lullo, E; Haeussler, M; Sandoval-Espinosa, C; Liu, SJ; Velmeshev, D; Ounadjela, JR; Shuga, J; Wang, X; Lim, DA; West, JA; Leyrat, AA; Kent, WJ; Kriegstein, AR

Published Date

  • December 8, 2017

Published In

Volume / Issue

  • 358 / 6368

Start / End Page

  • 1318 - 1323

PubMed ID

  • 29217575

Pubmed Central ID

  • PMC5991609

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

Digital Object Identifier (DOI)

  • 10.1126/science.aap8809

Language

  • eng

Conference Location

  • United States