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Neutrons do not produce a bystander effect in zebrafish irradiated in vivo.

Publication ,  Journal Article
Wang, C; Smith, RW; Duhig, J; Prestwich, WV; Byun, SH; McNeill, FE; Seymour, CB; Mothersill, CE
Published in: International journal of radiation biology
September 2011

Neutron irradiations at the McMaster Tandetron Accelerator were performed to study direct and bystander effects of neutrons in a live organism.The neutrons were produced through (7)Li(p,n)(7)Be reaction. Although the gamma contamination of the neutron beam cannot be completely eliminated, it was designed to be as low as possible and remain below a threshold already established for bystander effects. Microdosimetric methods using a tissue-equivalent proportional counter have been used to measure the neutron and gamma doses for the cell irradiation. Previous data for a cell line exposed in vitro suggested that neutrons did not produce bystander effects at doses below 300 mGy. The current experiments sought to confirm this using a live whole organism (zebrafish) where tissue samples harvested 2 h after exposure were examined for direct evidence of apoptosis and tested for secretion of bystander factors using an established bioassay. Fish were either exposed directly to the beam or were allowed to swim with or in water previously occupied by irradiated fish.Using the zebrafish model it was found that there was significant direct cell death seen both by apoptosis scores and clonogenic assay when the neutron dose was approximately 100 mGy. An equivalent dose of gamma rays produced a more toxic effect. It was further found that neutrons did not induce a bystander effect in fish receiving signals from irradiated fish.The results confirm in vitro experiments which suggest neutrons do not induce bystander signaling. In fact they may suppress gamma induced signaling suggesting a possible intriguing new and as yet unclear mechanism.

Duke Scholars

Published In

International journal of radiation biology

DOI

EISSN

1362-3095

ISSN

0955-3002

Publication Date

September 2011

Volume

87

Issue

9

Start / End Page

964 / 973

Related Subject Headings

  • Zebrafish
  • Stress, Physiological
  • Signal Transduction
  • Radiometry
  • Oncology & Carcinogenesis
  • Olfactory Mucosa
  • Neutrons
  • Male
  • Female
  • Cell Line
 

Citation

APA
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ICMJE
MLA
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Wang, C., Smith, R. W., Duhig, J., Prestwich, W. V., Byun, S. H., McNeill, F. E., … Mothersill, C. E. (2011). Neutrons do not produce a bystander effect in zebrafish irradiated in vivo. International Journal of Radiation Biology, 87(9), 964–973. https://doi.org/10.3109/09553002.2011.584939
Wang, Chu, Richard W. Smith, John Duhig, William V. Prestwich, Soo H. Byun, Fiona E. McNeill, Colin B. Seymour, and Carmel E. Mothersill. “Neutrons do not produce a bystander effect in zebrafish irradiated in vivo.International Journal of Radiation Biology 87, no. 9 (September 2011): 964–73. https://doi.org/10.3109/09553002.2011.584939.
Wang C, Smith RW, Duhig J, Prestwich WV, Byun SH, McNeill FE, et al. Neutrons do not produce a bystander effect in zebrafish irradiated in vivo. International journal of radiation biology. 2011 Sep;87(9):964–73.
Wang, Chu, et al. “Neutrons do not produce a bystander effect in zebrafish irradiated in vivo.International Journal of Radiation Biology, vol. 87, no. 9, Sept. 2011, pp. 964–73. Epmc, doi:10.3109/09553002.2011.584939.
Wang C, Smith RW, Duhig J, Prestwich WV, Byun SH, McNeill FE, Seymour CB, Mothersill CE. Neutrons do not produce a bystander effect in zebrafish irradiated in vivo. International journal of radiation biology. 2011 Sep;87(9):964–973.

Published In

International journal of radiation biology

DOI

EISSN

1362-3095

ISSN

0955-3002

Publication Date

September 2011

Volume

87

Issue

9

Start / End Page

964 / 973

Related Subject Headings

  • Zebrafish
  • Stress, Physiological
  • Signal Transduction
  • Radiometry
  • Oncology & Carcinogenesis
  • Olfactory Mucosa
  • Neutrons
  • Male
  • Female
  • Cell Line