Identification of distinct non-myogenic skeletal-muscle-resident mesenchymal cell populations.

Journal Article (Journal Article)

Mesenchymal progenitors of the lateral plate mesoderm give rise to various cell fates within limbs, including a heterogeneous group of muscle-resident mesenchymal cells. Often described as fibro-adipogenic progenitors, these cells are key players in muscle development, disease, and regeneration. To further define this cell population(s), we perform lineage/reporter analysis, flow cytometry, single-cell RNA sequencing, immunofluorescent staining, and differentiation assays on normal and injured murine muscles. Here we identify six distinct Pdgfra+ non-myogenic muscle-resident mesenchymal cell populations that fit within a bipartite differentiation trajectory from a common progenitor. One branch of the trajectory gives rise to two populations of immune-responsive mesenchymal cells with strong adipogenic potential and the capability to respond to acute and chronic muscle injury, whereas the alternative branch contains two cell populations with limited adipogenic capacity and inherent mineralizing capabilities; one of the populations displays a unique neuromuscular junction association and an ability to respond to nerve injury.

Full Text

Duke Authors

Cited Authors

  • Leinroth, AP; Mirando, AJ; Rouse, D; Kobayahsi, Y; Tata, PR; Rueckert, HE; Liao, Y; Long, JT; Chakkalakal, JV; Hilton, MJ

Published Date

  • May 10, 2022

Published In

Volume / Issue

  • 39 / 6

Start / End Page

  • 110785 -

PubMed ID

  • 35545045

Electronic International Standard Serial Number (EISSN)

  • 2211-1247

Digital Object Identifier (DOI)

  • 10.1016/j.celrep.2022.110785

Language

  • eng

Conference Location

  • United States