Systematic characterization of gene function in the photosynthetic alga Chlamydomonas reinhardtii.

Journal Article (Journal Article)

Most genes in photosynthetic organisms remain functionally uncharacterized. Here, using a barcoded mutant library of the model eukaryotic alga Chlamydomonas reinhardtii, we determined the phenotypes of more than 58,000 mutants under more than 121 different environmental growth conditions and chemical treatments. A total of 59% of genes are represented by at least one mutant that showed a phenotype, providing clues to the functions of thousands of genes. Mutant phenotypic profiles place uncharacterized genes into functional pathways such as DNA repair, photosynthesis, the CO2 -concentrating mechanism and ciliogenesis. We illustrate the value of this resource by validating phenotypes and gene functions, including three new components of an actin cytoskeleton defense pathway. The data also inform phenotype discovery in land plants; mutants in Arabidopsis thaliana genes exhibit phenotypes similar to those we observed in their Chlamydomonas homologs. We anticipate that this resource will guide the functional characterization of genes across the tree of life.

Full Text

Duke Authors

Cited Authors

  • Fauser, F; Vilarrasa-Blasi, J; Onishi, M; Ramundo, S; Patena, W; Millican, M; Osaki, J; Philp, C; Nemeth, M; Salomé, PA; Li, X; Wakao, S; Kim, RG; Kaye, Y; Grossman, AR; Niyogi, KK; Merchant, SS; Cutler, SR; Walter, P; Dinneny, JR; Jonikas, MC; Jinkerson, RE

Published Date

  • May 2022

Published In

Volume / Issue

  • 54 / 5

Start / End Page

  • 705 - 714

PubMed ID

  • 35513725

Pubmed Central ID

  • PMC9110296

Electronic International Standard Serial Number (EISSN)

  • 1546-1718

International Standard Serial Number (ISSN)

  • 1061-4036

Digital Object Identifier (DOI)

  • 10.1038/s41588-022-01052-9


  • eng