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Gaseous air pollutants and DNA methylation in a methylome-wide association study of an ethnically and environmentally diverse population of U.S. adults.

Publication ,  Journal Article
Holliday, KM; Gondalia, R; Baldassari, A; Justice, AE; Stewart, JD; Liao, D; Yanosky, JD; Jordahl, KM; Bhatti, P; Assimes, TL; Pankow, JS ...
Published in: Environ Res
September 2022

Epigenetic mechanisms may underlie air pollution-health outcome associations. We estimated gaseous air pollutant-DNA methylation (DNAm) associations using twelve subpopulations within Women's Health Initiative (WHI) and Atherosclerosis Risk in Communities (ARIC) cohorts (n = 8397; mean age 61.3 years; 83% female; 46% African-American, 46% European-American, 8% Hispanic/Latino). We used geocoded participant address-specific mean ambient carbon monoxide (CO), nitrogen oxides (NO2; NOx), ozone (O3), and sulfur dioxide (SO2) concentrations estimated over the 2-, 7-, 28-, and 365-day periods before collection of blood samples used to generate Illumina 450 k array leukocyte DNAm measurements. We estimated methylome-wide, subpopulation- and race/ethnicity-stratified pollutant-DNAm associations in multi-level, linear mixed-effects models adjusted for sociodemographic, behavioral, meteorological, and technical covariates. We combined stratum-specific estimates in inverse variance-weighted meta-analyses and characterized significant associations (false discovery rate; FDR<0.05) at Cytosine-phosphate-Guanine (CpG) sites without among-strata heterogeneity (PCochran's Q > 0.05). We attempted replication in the Cooperative Health Research in Region of Augsburg (KORA) study and Normative Aging Study (NAS). We observed a -0.3 (95% CI: -0.4, -0.2) unit decrease in percent DNAm per interquartile range (IQR, 7.3 ppb) increase in 28-day mean NO2 concentration at cg01885635 (chromosome 3; regulatory region 290 bp upstream from ZNF621; FDR = 0.03). At intragenic sites cg21849932 (chromosome 20; LIME1; intron 3) and cg05353869 (chromosome 11; KLHL35; exon 2), we observed a -0.3 (95% CI: -0.4, -0.2) unit decrease (FDR = 0.04) and a 1.2 (95% CI: 0.7, 1.7) unit increase (FDR = 0.04), respectively, in percent DNAm per IQR (17.6 ppb) increase in 7-day mean ozone concentration. Results were not fully replicated in KORA and NAS. We identified three CpG sites potentially susceptible to gaseous air pollution-induced DNAm changes near genes relevant for cardiovascular and lung disease. Further harmonized investigations with a range of gaseous pollutants and averaging durations are needed to determine the effect of gaseous air pollutants on DNA methylation and ultimately gene expression.

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Published In

Environ Res

DOI

EISSN

1096-0953

Publication Date

September 2022

Volume

212

Issue

Pt C

Start / End Page

113360

Location

Netherlands

Related Subject Headings

  • Toxicology
  • Particulate Matter
  • Ozone
  • Nitrogen Dioxide
  • Middle Aged
  • Male
  • Humans
  • Female
  • Epigenome
  • DNA Methylation
 

Citation

APA
Chicago
ICMJE
MLA
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Holliday, K. M., Gondalia, R., Baldassari, A., Justice, A. E., Stewart, J. D., Liao, D., … Whitsel, E. A. (2022). Gaseous air pollutants and DNA methylation in a methylome-wide association study of an ethnically and environmentally diverse population of U.S. adults. Environ Res, 212(Pt C), 113360. https://doi.org/10.1016/j.envres.2022.113360
Holliday, Katelyn M., Rahul Gondalia, Antoine Baldassari, Anne E. Justice, James D. Stewart, Duanping Liao, Jeff D. Yanosky, et al. “Gaseous air pollutants and DNA methylation in a methylome-wide association study of an ethnically and environmentally diverse population of U.S. adults.Environ Res 212, no. Pt C (September 2022): 113360. https://doi.org/10.1016/j.envres.2022.113360.
Holliday KM, Gondalia R, Baldassari A, Justice AE, Stewart JD, Liao D, et al. Gaseous air pollutants and DNA methylation in a methylome-wide association study of an ethnically and environmentally diverse population of U.S. adults. Environ Res. 2022 Sep;212(Pt C):113360.
Holliday, Katelyn M., et al. “Gaseous air pollutants and DNA methylation in a methylome-wide association study of an ethnically and environmentally diverse population of U.S. adults.Environ Res, vol. 212, no. Pt C, Sept. 2022, p. 113360. Pubmed, doi:10.1016/j.envres.2022.113360.
Holliday KM, Gondalia R, Baldassari A, Justice AE, Stewart JD, Liao D, Yanosky JD, Jordahl KM, Bhatti P, Assimes TL, Pankow JS, Guan W, Fornage M, Bressler J, North KE, Conneely KN, Li Y, Hou L, Vokonas PS, Ward-Caviness CK, Wilson R, Wolf K, Waldenberger M, Cyrys J, Peters A, Boezen HM, Vonk JM, Sayols-Baixeras S, Lee M, Baccarelli AA, Whitsel EA. Gaseous air pollutants and DNA methylation in a methylome-wide association study of an ethnically and environmentally diverse population of U.S. adults. Environ Res. 2022 Sep;212(Pt C):113360.
Journal cover image

Published In

Environ Res

DOI

EISSN

1096-0953

Publication Date

September 2022

Volume

212

Issue

Pt C

Start / End Page

113360

Location

Netherlands

Related Subject Headings

  • Toxicology
  • Particulate Matter
  • Ozone
  • Nitrogen Dioxide
  • Middle Aged
  • Male
  • Humans
  • Female
  • Epigenome
  • DNA Methylation