Mucor circinelloides: Growth, Maintenance, and Genetic Manipulation.
Publication
, Journal Article
Vellanki, S; Navarro-Mendoza, MI; Garcia, A; Murcia, L; Perez-Arques, C; Garre, V; Nicolas, FE; Lee, SC
Published in: Current protocols in microbiology
May 2018
Mucor circinelloides is a fungus that belongs to the order Mucorales. It grows as mold in the environment and can cause mucormycosis, a potentially fatal infection in immunocompromised patients. M. circinelloides is a biodiesel producer and serves as a model organism for studying several biological processes, such as light responses and RNA interference-mediated gene silencing. Over the past decade, the increasing number of molecular tools has also allowed us to manipulate the genome of this fungus. This article outlines the fundamental protocols for the in vitro growth, maintenance, and genetic manipulation of M. circinelloides in the laboratory. © 2018 by John Wiley & Sons, Inc.
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Published In
Current protocols in microbiology
DOI
EISSN
1934-8533
ISSN
1934-8525
Publication Date
May 2018
Volume
49
Issue
1
Start / End Page
e53
Related Subject Headings
- Spores, Fungal
- Sequence Deletion
- Preservation, Biological
- Polymerase Chain Reaction
- Mucormycosis
- Mucor
- Microbiology
- Humans
- Genetic Vectors
- Gene Silencing
Citation
APA
Chicago
ICMJE
MLA
NLM
Vellanki, S., Navarro-Mendoza, M. I., Garcia, A., Murcia, L., Perez-Arques, C., Garre, V., … Lee, S. C. (2018). Mucor circinelloides: Growth, Maintenance, and Genetic Manipulation. Current Protocols in Microbiology, 49(1), e53. https://doi.org/10.1002/cpmc.53
Vellanki, Sandeep, Maria Isabel Navarro-Mendoza, Alexis Garcia, Laura Murcia, Carlos Perez-Arques, Victoriano Garre, Francisco E. Nicolas, and Soo Chan Lee. “Mucor circinelloides: Growth, Maintenance, and Genetic Manipulation.” Current Protocols in Microbiology 49, no. 1 (May 2018): e53. https://doi.org/10.1002/cpmc.53.
Vellanki S, Navarro-Mendoza MI, Garcia A, Murcia L, Perez-Arques C, Garre V, et al. Mucor circinelloides: Growth, Maintenance, and Genetic Manipulation. Current protocols in microbiology. 2018 May;49(1):e53.
Vellanki, Sandeep, et al. “Mucor circinelloides: Growth, Maintenance, and Genetic Manipulation.” Current Protocols in Microbiology, vol. 49, no. 1, May 2018, p. e53. Epmc, doi:10.1002/cpmc.53.
Vellanki S, Navarro-Mendoza MI, Garcia A, Murcia L, Perez-Arques C, Garre V, Nicolas FE, Lee SC. Mucor circinelloides: Growth, Maintenance, and Genetic Manipulation. Current protocols in microbiology. 2018 May;49(1):e53.
Published In
Current protocols in microbiology
DOI
EISSN
1934-8533
ISSN
1934-8525
Publication Date
May 2018
Volume
49
Issue
1
Start / End Page
e53
Related Subject Headings
- Spores, Fungal
- Sequence Deletion
- Preservation, Biological
- Polymerase Chain Reaction
- Mucormycosis
- Mucor
- Microbiology
- Humans
- Genetic Vectors
- Gene Silencing