Visualizing cytoplasmic ATP in C. elegans larvae using PercevalHR.

Journal Article (Journal Article)

Measuring ATP levels within the cytosol of living cells in animals is important to understand how cellular activities are energetically supported, but is challenging because of tissue complexity and ATP sensor limitations. In this protocol, we describe how to quantify ATP levels using PercevalHR in C. elegans larvae during anchor cell invasion. PercevalHR is a fluorescent biosensor that reports the cytoplasmic ATP:ADP ratio. The protocol can be adapted to analyze the ATP:ADP ratios within other cell types in C. elegans . For complete details on the use and execution of this protocol, please refer to Garde et al. (2022).

Full Text

Duke Authors

Cited Authors

  • Garde, A; Sherwood, DR

Published Date

  • June 2022

Published In

Volume / Issue

  • 3 / 2

Start / End Page

  • 101429 -

PubMed ID

  • 35664256

Pubmed Central ID

  • PMC9157558

Electronic International Standard Serial Number (EISSN)

  • 2666-1667

International Standard Serial Number (ISSN)

  • 2666-1667

Digital Object Identifier (DOI)

  • 10.1016/j.xpro.2022.101429

Language

  • eng