Visualizing cytoplasmic ATP in C. elegans larvae using PercevalHR.
Publication
, Journal Article
Garde, A; Sherwood, DR
Published in: STAR protocols
June 2022
Measuring ATP levels within the cytosol of living cells in animals is important to understand how cellular activities are energetically supported, but is challenging because of tissue complexity and ATP sensor limitations. In this protocol, we describe how to quantify ATP levels using PercevalHR in C. elegans larvae during anchor cell invasion. PercevalHR is a fluorescent biosensor that reports the cytoplasmic ATP:ADP ratio. The protocol can be adapted to analyze the ATP:ADP ratios within other cell types in C. elegans. For complete details on the use and execution of this protocol, please refer to Garde et al. (2022).
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Published In
STAR protocols
DOI
EISSN
2666-1667
ISSN
2666-1667
Publication Date
June 2022
Volume
3
Issue
2
Start / End Page
101429
Related Subject Headings
- Larva
- Cytosol
- Caenorhabditis elegans
- Animals
- Adenosine Triphosphate
- Adenosine Diphosphate
Citation
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Garde, A., & Sherwood, D. R. (2022). Visualizing cytoplasmic ATP in C. elegans larvae using PercevalHR. STAR Protocols, 3(2), 101429. https://doi.org/10.1016/j.xpro.2022.101429
Garde, Aastha, and David R. Sherwood. “Visualizing cytoplasmic ATP in C. elegans larvae using PercevalHR.” STAR Protocols 3, no. 2 (June 2022): 101429. https://doi.org/10.1016/j.xpro.2022.101429.
Garde A, Sherwood DR. Visualizing cytoplasmic ATP in C. elegans larvae using PercevalHR. STAR protocols. 2022 Jun;3(2):101429.
Garde, Aastha, and David R. Sherwood. “Visualizing cytoplasmic ATP in C. elegans larvae using PercevalHR.” STAR Protocols, vol. 3, no. 2, June 2022, p. 101429. Epmc, doi:10.1016/j.xpro.2022.101429.
Garde A, Sherwood DR. Visualizing cytoplasmic ATP in C. elegans larvae using PercevalHR. STAR protocols. 2022 Jun;3(2):101429.
Published In
STAR protocols
DOI
EISSN
2666-1667
ISSN
2666-1667
Publication Date
June 2022
Volume
3
Issue
2
Start / End Page
101429
Related Subject Headings
- Larva
- Cytosol
- Caenorhabditis elegans
- Animals
- Adenosine Triphosphate
- Adenosine Diphosphate