Charged residues on the side of the nucleosome contribute to normal Spt16-gene interactions in budding yeast.

Journal Article (Journal Article)

Previous work in Saccharomyces cerevisiae identified three residues located in close proximity to each other on the side of the nucleosome whose integrity is required for proper association of the Spt16 component of the FACT complex across transcribed genes. In an effort to gain further insights into the parameters that control Spt16 interactions with genes in vivo, we tested the effects of additional histone mutants on Spt16 occupancy across two constitutively transcribed genes. These studies revealed that mutations in several charged residues in the vicinity of the three residues originally identified as important for Spt16-gene interactions also significantly perturb normal association of Spt16 across genes. Based on these and our previous findings, we propose that the charge landscape across the region encompassed by these residues, which we refer to as the Influences Spt16-Gene Interactions or ISGI region, is an important contributor to proper Spt16-gene interactions in vivo.

Full Text

Duke Authors

Cited Authors

  • Nyamugenda, E; Cox, AB; Pierce, JB; Banning, RC; Huynh, ML; May, C; Marshall, S; Turkal, CE; Duina, AA

Published Date

  • January 2018

Published In

Volume / Issue

  • 13 / 1

Start / End Page

  • 1 - 7

PubMed ID

  • 29271283

Pubmed Central ID

  • PMC5836983

Electronic International Standard Serial Number (EISSN)

  • 1559-2308

International Standard Serial Number (ISSN)

  • 1559-2294

Digital Object Identifier (DOI)

  • 10.1080/15592294.2017.1418132


  • eng