Immune synapses between mast cells and γδ T cells limit viral infection.
Mast cells (MCs) are immune sentinels, but whether they also function as antigen-presenting cells (APCs) remains elusive. Using mouse models of MC deficiency, we report on MC-dependent recruitment and activation of multiple T cell subsets to the skin and draining lymph nodes (DLNs) during dengue virus (DENV) infection. Newly recruited and locally proliferating γδ T cells were the first T cell subset to respond to MC-driven inflammation, and their production of IFN-γ was MC dependent. MC-γδ T cell conjugates were observed consistently in infected peripheral tissues, suggesting a new role for MCs as nonconventional APCs for γδ T cells. MC-dependent γδ T cell activation and proliferation during DENV infection required T cell receptor (TCR) signaling and the nonconventional antigen presentation molecule endothelial cell protein C receptor (EPCR) on MCs. γδ T cells, not previously implicated in DENV host defense, killed infected targeted DCs and contributed to the clearance of DENV in vivo. We believe immune synapse formation between MCs and γδ T cells is a novel mechanism to induce specific and protective immunity at sites of viral infection.
Duke Scholars
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Related Subject Headings
- T-Lymphocytes
- Skin
- Receptors, Antigen, T-Cell, gamma-delta
- Mice, Transgenic
- Mice
- Mast Cells
- Immunology
- Immunological Synapses
- Endothelial Protein C Receptor
- Disease Models, Animal
Citation
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- T-Lymphocytes
- Skin
- Receptors, Antigen, T-Cell, gamma-delta
- Mice, Transgenic
- Mice
- Mast Cells
- Immunology
- Immunological Synapses
- Endothelial Protein C Receptor
- Disease Models, Animal