Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures.
Journal Article (Journal Article)
Alveolar type 2 cells (AT2s) serve as stem cells of the alveoli and restore cell numbers after injury. Here, we describe a detailed protocol for the isolation, purification, and culture of murine and human AT2s. We have developed chemically defined and stroma-free culture conditions that enable expansion and maintenance of AT2s. The culture conditions are scalable and compatible with high-throughput chemical and genetic screenings and can potentially be used to generate large AT2 numbers for cell-based therapies. For complete details on the use and execution of this protocol, please refer to Katsura et al. (2020).
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Duke Authors
Cited Authors
- Konishi, S; Tata, A; Tata, PR
Published Date
- June 17, 2022
Published In
Volume / Issue
- 3 / 2
Start / End Page
- 101447 -
PubMed ID
- 35712012
Pubmed Central ID
- PMC9192963
Electronic International Standard Serial Number (EISSN)
- 2666-1667
Digital Object Identifier (DOI)
- 10.1016/j.xpro.2022.101447
Language
- eng
Conference Location
- United States