Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures.

Journal Article (Journal Article)

Alveolar type 2 cells (AT2s) serve as stem cells of the alveoli and restore cell numbers after injury. Here, we describe a detailed protocol for the isolation, purification, and culture of murine and human AT2s. We have developed chemically defined and stroma-free culture conditions that enable expansion and maintenance of AT2s. The culture conditions are scalable and compatible with high-throughput chemical and genetic screenings and can potentially be used to generate large AT2 numbers for cell-based therapies. For complete details on the use and execution of this protocol, please refer to Katsura et al. (2020).

Full Text

Duke Authors

Cited Authors

  • Konishi, S; Tata, A; Tata, PR

Published Date

  • June 17, 2022

Published In

Volume / Issue

  • 3 / 2

Start / End Page

  • 101447 -

PubMed ID

  • 35712012

Pubmed Central ID

  • PMC9192963

Electronic International Standard Serial Number (EISSN)

  • 2666-1667

Digital Object Identifier (DOI)

  • 10.1016/j.xpro.2022.101447


  • eng

Conference Location

  • United States