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Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures.

Publication ,  Journal Article
Konishi, S; Tata, A; Tata, PR
Published in: STAR Protoc
June 17, 2022

Alveolar type 2 cells (AT2s) serve as stem cells of the alveoli and restore cell numbers after injury. Here, we describe a detailed protocol for the isolation, purification, and culture of murine and human AT2s. We have developed chemically defined and stroma-free culture conditions that enable expansion and maintenance of AT2s. The culture conditions are scalable and compatible with high-throughput chemical and genetic screenings and can potentially be used to generate large AT2 numbers for cell-based therapies. For complete details on the use and execution of this protocol, please refer to Katsura et al. (2020).

Duke Scholars

Published In

STAR Protoc

DOI

EISSN

2666-1667

Publication Date

June 17, 2022

Volume

3

Issue

2

Start / End Page

101447

Location

United States

Related Subject Headings

  • Stem Cells
  • Pulmonary Alveoli
  • Mice
  • Lung
  • Humans
  • Cell Differentiation
  • Animals
  • Alveolar Epithelial Cells
 

Citation

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Chicago
ICMJE
MLA
NLM
Konishi, S., Tata, A., & Tata, P. R. (2022). Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures. STAR Protoc, 3(2), 101447. https://doi.org/10.1016/j.xpro.2022.101447
Konishi, Satoshi, Aleksandra Tata, and Purushothama Rao Tata. “Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures.STAR Protoc 3, no. 2 (June 17, 2022): 101447. https://doi.org/10.1016/j.xpro.2022.101447.
Konishi, Satoshi, et al. “Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures.STAR Protoc, vol. 3, no. 2, June 2022, p. 101447. Pubmed, doi:10.1016/j.xpro.2022.101447.
Journal cover image

Published In

STAR Protoc

DOI

EISSN

2666-1667

Publication Date

June 17, 2022

Volume

3

Issue

2

Start / End Page

101447

Location

United States

Related Subject Headings

  • Stem Cells
  • Pulmonary Alveoli
  • Mice
  • Lung
  • Humans
  • Cell Differentiation
  • Animals
  • Alveolar Epithelial Cells